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Monoterpene separation by coupling proton transfer reaction time-of-flight mass spectrometry with fastGC

Materić, Dušan; Lanza, Matteo; Sulzer, Philipp; Herbig, Jens; Bruhn, Dan; Turner, Claire; Mason, Nigel and Gauci, Vincent (2015). Monoterpene separation by coupling proton transfer reaction time-of-flight mass spectrometry with fastGC. Analytical and Bioanalytical Chemistry, 407(25) pp. 7757–7763.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1007/s00216-015-8942-5
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Abstract

Proton Transfer Reaction Mass Spectrometry (PTR-MS) is a well-established technique for real-time VOCs (Volatile Organic Compounds) analysis. Although, it is extremely sensitive (with sensitivities of up to 4500 cps/ppbv, limits of the detection < 1 pptv and the response times of approximately 100 ms) the selectivity of PTR-MS is still somewhat limited, as isomers cannot be separated. Recently, selectivity-enhancing measures, such as manipulation of drift tube parameters (reduced electric field strength) and using primary ions other than H3O+, such as NO+ and O2+ have been introduced. However, monoterpenes, which belong to the most important plant VOCs, still cannot be distinguished so that more traditional technologies, such as gas chromatography mass spectrometry (GC-MS), have to be utilized. GC-MS is very time consuming (up to 1 h) and cannot be used for real-time analysis. Here we introduce a sensitive, near real-time method for plant monoterpene research: PTR-MS coupled with fastGC. We successfully separated and identified six of the most abundant monoterpenes in plant studies (α- and β-pinenes, limonene, 3-carene, camphene, and myrcene) in less than 80 s, using both standards and conifer branch enclosures (Norway spruce, Scots pine and Black pine). Five monoterpenes usually present in Norway spruce samples with a high abundance were separated even when the compound concentrations were diluted to 20 ppbv. Thus, fastGC-PTR-ToF-MS was shown to be an adequate one-instrument solution for plant monoterpene research.

Item Type: Journal Item
Copyright Holders: 2015 Springer-Verlag Berlin Heidelberg
ISSN: 1618-2642
Project Funding Details:
Funded Project NameProject IDFunding Body
Proton Ionization Molecular Mass Spectrometry (PIMMS) Initial Training Network (ITN)287382EU FP7 Programme
Keywords: PTR-MS; fastGC; monoterpenes; VOC; plant VOCs; pinene
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Environment, Earth and Ecosystem Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Centre for Earth, Planetary, Space and Astronomical Research (CEPSAR)
Innovation, Knowledge & Development research centre (IKD)
OpenSpace Research Centre (OSRC)
International Development & Inclusive Innovation
Item ID: 44016
Depositing User: Dušan Materić
Date Deposited: 14 Aug 2015 10:46
Last Modified: 26 May 2017 12:19
URI: http://oro.open.ac.uk/id/eprint/44016
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