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Pulmonary vein sleeve cell excitation–contraction-coupling becomes dysynchronized by spontaneous calcium transients

Rietdorf, Katja; Masoud, Said; McDonald, Fraser; Sanderson, Michael J. and Bootman, Martin D. (2015). Pulmonary vein sleeve cell excitation–contraction-coupling becomes dysynchronized by spontaneous calcium transients. Biochemical Society Transactions, 43(3) pp. 410–416.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1042/BST20140299
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Abstract

Atrial fibrillation (AF) is the most common form of sustained cardiac arrhythmia. Substantial evidence indicates that cardiomyocytes located in the pulmonary veins [pulmonary vein sleeve cells (PVCs)] cause AF by generating ectopic electrical activity. Electrical ablation, isolating PVCs from their left atrial junctions, is a major treatment for AF. In small rodents, the sleeve of PVCs extends deep inside the lungs and is present in lung slices. Here we present data, using the lung slice preparation, characterizing how spontaneous Ca2+ transients in PVCs affect their capability to respond to electrical pacing. Immediately after a spontaneous Ca2+ transient the cell is in a refractory period and it cannot respond to electrical stimulation. Consequently, we observe that the higher the level of spontaneous activity in an individual PVC, the less likely it is that this PVC responds to electrical field stimulation. The spontaneous activity of neighbouring PVCs can be different from each other. Heterogeneity in the Ca2+ signalling of cells and in their responsiveness to electrical stimuli are known pro-arrhythmic events. The tendency of PVCs to show spontaneous Ca2+ transients and spontaneous action potentials (APs) underlies their potential to cause AF.

Item Type: Journal Item
Copyright Holders: 2015 The Authors
ISSN: 1470-8752
Keywords: atrial fibrillation; calcium signalling; cardiac arrhythmia; lung slice; pulmonary vein; myocyte
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Health and Wellbeing PRA (Priority Research Area)
Item ID: 43919
Depositing User: Martin Bootman
Date Deposited: 12 Aug 2015 08:37
Last Modified: 11 Sep 2017 15:32
URI: http://oro.open.ac.uk/id/eprint/43919
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