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Systems for measuring B cell receptor affinity maturation in germinal centres

Müller, Thomas; Marshall, Jennifer; Dehghani, Hamid; Bowen, James and Toellner, Kai-Michael (2014). Systems for measuring B cell receptor affinity maturation in germinal centres. In: ID2 Inter-Disciplinary Inter-DTC, 26-27 June 2014, Oxford, UK.

URL: https://www.academia.edu/9854637/Systems_for_measu...
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Abstract

In an effort to improve our understanding of the germinal centre (GC), this project aims to measure affinities of B cell receptors through the course of an immune response in order to map out spatial relationships of individual B cells during evolution in the GC. For this atomic force microscopy (AFM) and flow cytometry are being applied. Modifications to the AFM setup have been made to provide specificity towards the targeted receptors, and work on a range of known affinity B cells will demonstrate whether differences in affinities are distinguishable via AFM. Bespoke fluorescent probes have been prepared for use in flow cytometry to allow following affinity maturation during an immune response in large cell populations providing measures of relative affinity. When applied in histology this will also provide spatial information.

Item Type: Conference or Workshop Item
Copyright Holders: 2014 The Authors
Keywords: biomedical engineering; flow cytometry; atomic force microscopy; adhesion; infection; immunity
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
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Item ID: 43387
Depositing User: James Bowen
Date Deposited: 09 Jun 2015 08:36
Last Modified: 10 Nov 2016 17:18
URI: http://oro.open.ac.uk/id/eprint/43387
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