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A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface

Koburger, Susanne; Bannerman, Alistair; Grover, Liam M.; Müller, Frank A.; Bowen, James and Paxton, Jennifer (2014). A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface. Biomaterials Science, 2(1) pp. 41–51.

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The capacity to study the deposition of mineral within a hydrogel structure is of significant interest to a range of therapies that seek to replace the hard tissues and the hard–soft tissue interface. Here, a method is presented that utilises Confocal Raman microscopy as a tool for monitoring mineralisation within hydrogels. Synthetic hard–soft material interfaces were fabricated by apposing brushite (a sparingly soluble calcium phosphate) and biopolymer gel monoliths. The resulting structures were matured over a period of 28 days in phosphate buffered saline. Confocal Raman microscopy of the interfacial region showed the appearance of calcium phosphate salt deposits away from the original interface within the biopolymeric structures. Furthermore, the appearance of octacalcium phosphate and carbonated hydroxyapatite was observed in the region of the brushite cement opposing the biopolymer gel. This study describes not only a method for analysing these composite structures, but also suggests a method for recapitulating the graduated tissue structures that are often found in vivo.

Item Type: Journal Item
Copyright Holders: 2014 The Royal Society of Chemistry
ISSN: 2047-4849
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
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Item ID: 43158
Depositing User: James Bowen
Date Deposited: 27 May 2015 15:13
Last Modified: 08 Dec 2018 15:44
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