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Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Piletsky, Sergey; Piletska, Elena; Bossi, Alessandra; Turner, Nicholas and Turner, Anthony (2003). Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization. Biotechnology and Bioengineering, 82(1) pp. 86–92.

DOI (Digital Object Identifier) Link: https://doi.org/10.1002/bit.10544
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Abstract

Commercial porous polypropylene membranes were chemically modified with polyaniline (PANI) using ammonium persulfate as the oxidizer. The influence of polymerization conditions on the membrane properties was studied by adsorption analysis and membrane permeability. The PANI-coated polypropylene (PANI/PP) membranes possessed high affinity toward the proteins, which can be immobilized onto the membrane surface through physical adsorption or covalent immobilization. The quantity of immobilized horseradish peroxidase (HRP) and its activity depended on the quantity and quality (oxidation level) of PANI. The storage conditions for PANI/PP membranes containing immobilized HRP were studied. HRP immobilized on the PANI/PP membrane was shown to retain 70% of its activity after 3-month storage at +5°C, suggesting that this material can be used for practical application, such as in bioreactors as enzyme membranes.

Item Type: Journal Item
Copyright Holders: 2003 Wiley Periodicals, Inc.
ISSN: 1097-0290
Keywords: coating; polyaniline; membranes; filtration; affinity separation
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 41998
Depositing User: Nicholas Turner
Date Deposited: 18 Feb 2015 11:38
Last Modified: 07 Dec 2018 10:28
URI: http://oro.open.ac.uk/id/eprint/41998
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