The Open UniversitySkip to content
 

Trifluorosilane induced structural transitions in beta-lactoglobulin in sol and gel

Peng, Yun; Turner, Nicholas W. and Britt, David W. (2014). Trifluorosilane induced structural transitions in beta-lactoglobulin in sol and gel. Colloids and Surfaces B: Biointerfaces, 119 pp. 6–13.

DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.colsurfb.2014.04.015
Google Scholar: Look up in Google Scholar

Abstract

Fluoroalcohols such as 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) are strong inducers of protein secondary structure. Here, hydrolyzed (3,3,3-trifluoropropyl)trimethoxysilane (3F) is demonstrated to exhibit greater protein conformation inducing activity than HFIP, which is preserved when 3F is copolymerized in tetraethylorthosilicate (TEOS) sol–gels through protein molecular imprinting. Hydrolyzed 3F formed a pre-polymerization complex with the template protein, β-lactoglobulin, inducing distinct α-helical structures as evidenced by circular dichroism. Fluorescence resonance energy transfer between tryptophan and the lipophilic probe 1-anilinonaphthalene-8-sulfonic acid showed a sharp molten globule (MG) transition at 0.2 M 3F, whereas HFIP induced a broad MG transition centered at 0.6 M HFIP. The 3F-induced BLG conformation transitions were retained upon gelation, validating use of the fluorosilane as a conformation directing functional monomer readily incorporated into sol–gels.

Item Type: Journal Item
Copyright Holders: 2014 Elsevier B.V.
ISSN: 0927-7765
Keywords: functional monomer; fluorosilane; fluoroalcohol; β-lactoglobulin; sol–gel encapsulation; protein conformation transition
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 41990
Depositing User: Nicholas Turner
Date Deposited: 04 Feb 2015 14:19
Last Modified: 07 Dec 2018 10:28
URI: http://oro.open.ac.uk/id/eprint/41990
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU