Zulfiqar, Adnan; Morgan, Geraint and Turner, Nicholas W.
(2014).
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1039/c4an00721b |
|---|---|
| Google Scholar: | Look up in Google Scholar |
Abstract
A method capable of screening for multiple steroids in urine has been developed, using a series of twelve structurally similar, and commercially relevant compounds as target analytes. A molecularly imprinted solid phase extraction clean-up step was used to make the sample suitable for injection onto a GC×GC-MS setup. Significant improvements compared to a commercially available C-18 material were observed. Each individual steroid was able to be separated and identified, using both the retention profile and diagnostic fragmentation ion monitoring abilities of the comprehensive chromatographic-mass spectrometry method. Effective LODs of between 11.7 and 27.0 pg were calculated for individual steroids, effectively equivalent to concentration levels of between 0.234 and 0.540 ng mL-1 in urine, while the application of multiple screen was demonstrated using a 10 ng mL-1 mixed sample. The nature of this study also removes the need for sample derivitisation which speeds up the screening process.
| Item Type: | Journal Item |
|---|---|
| Copyright Holders: | 2014 The Royal Society of Chemistry |
| ISSN: | 1364-5528 |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Item ID: | 41988 |
| Depositing User: | Nicholas Turner |
| Date Deposited: | 09 Feb 2015 12:08 |
| Last Modified: | 07 Dec 2018 10:28 |
| URI: | http://oro.open.ac.uk/id/eprint/41988 |
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