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Monitoring neuron and astrocyte interactions with a 3D cell culture system

Phillips, James B. (2014). Monitoring neuron and astrocyte interactions with a 3D cell culture system. In: Murray, Andrew J. ed. Axon Growth and Regeneration: Methods and Regeneration. Methods in Molecular Biology: Methods and Protocols, 1162. New York: Springer, pp. 113–124.

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Methods are described for the generation and analysis of 3D co-culture models in which astrocyte and neuronal behavior can be studied. Cells may be obtained from a variety of sources, then cultured within collagen hydrogels to explore cellular responses and interactions in response to substances under test or under conditions that mimic physiological or pathological environments. Cell populations are labelled then either mixed within gels or arranged as separate adjacent populations, with further options including directing the self-alignment of cells to form anisotropic 3D cultures. Immunofluorescence staining and confocal microscopy can be used to capture image data from 3D structures and detailed protocols are provided for obtaining reliable results. Finally, 3D image analysis of confocal microscopy data is discussed, providing guidance on how astrocyte and neuronal features can be quantified.

Item Type: Book Section
Copyright Holders: 2014 Springer Science+Business Media New York
ISBN: 1-4939-0776-X, 978-1-4939-0776-2
Keywords: neuron; astrocyte; neurite; 3D cell culture; confocal microscopy; collagen hydrogel; neural tissue engineering; reactive gliosis
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
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Item ID: 40682
Depositing User: James Phillips
Date Deposited: 06 Aug 2014 10:52
Last Modified: 04 Oct 2016 11:37
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