Phillips, J.B.; Bunting, S.C.J.; Ward, Z.; Hall, S.M. and Brown, R.A.
(2003).
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1109/MCTE.2002.1175057 |
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| Google Scholar: | Look up in Google Scholar |
Abstract
Fibronectin mats have proved to be successful guidance substrates for the outgrowth of neurites in models of neural damage. The overall aim of this investigation was to develop a tissue engineering conduit for surgical nerve reconstruction. In this study, fibronectin tubes were developed to provide global guidance for neural regrowth across a short gap. A viscous solution of concentrated plasma fibronectin was obtained from by-products of the human plasma fractionation process. The tube was formed by winding this material around an 18G hypodermic needle and allowing it to dry. The structure and physical properties of the tube were investigated. The spiral-wound material on the needles fused to form a durable tube, whose lumen remained patent after rehydration/swelling in buffer (swelling and stability were characterized). In vivo testing showed growth of axons through the tube 6 weeks post surgery in a rat sciatic nerve injury model. In conclusion, a tube has been developed from fibronectin which can be used to bridge gaps in damaged nerves. This could form the basis for the construction of a surgically implantable device for the tissue engineered repair of peripheral nerve injury.
| Item Type: | Conference or Workshop Item |
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| Extra Information: | pp. 165-166 of Proceedings. |
| Keywords: | biomedical equipment; neurophysiology; proteins; surgery |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Interdisciplinary Research Centre: | Biomedical Research Network (BRN) |
| Item ID: | 4065 |
| Depositing User: | James Phillips |
| Date Deposited: | 20 Sep 2006 |
| Last Modified: | 04 Oct 2016 09:51 |
| URI: | http://oro.open.ac.uk/id/eprint/4065 |
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