The Open UniversitySkip to content
 

A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

Kress, Horst; Jarrin, Andres; Thuroff, Eduardo; Saunders, Robert; Weise, Chris; Scmidt am Busch, Marcel; Knapp, Ernst-W.; Wedde, Marianne and Vilcinskas, Andreas (2004). A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation. Insect Biochemistry and Molecular Biology, 34(8) pp. 855–869.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1016/j.ibmb.2004.05.006
Google Scholar: Look up in Google Scholar

Abstract

From the Drosophila virilis late puff region 31C, we microcloned two neighbouring genes, Kil-1 and Kil-2, that encode putative Kunitz serine protease inhibitor like proteins. The Kil-1 gene is expressed exclusively in prepupal salivary glands. Using a size mutant of the KIL-1 protein and MALDI-TOF analysis, we demonstrate that during pupation this protein is released from the prepupal salivary glands into the pupation fluid covering the surface of the pupa. 3-D-structure predictions are consistent with the known crystal structure of the human Kunitz type protease inhibitor 2KNT. This is the first experimental proof for the extracorporal presence of a distinct Drosophila prepupal salivary gland protein. Possible functions of KIL-1 in the context of the control of proteolytic activities in the pupation fluid are discussed.

Item Type: Journal Article
ISSN: 0965-1748
Keywords: Drosophila; prepupal salivary gland; Kunitz serine protease inhibitor; Ecdysone; metamorphosis; pupation fluid
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 4029
Depositing User: Robert Saunders
Date Deposited: 06 Jul 2006
Last Modified: 10 Mar 2014 09:28
URI: http://oro.open.ac.uk/id/eprint/4029
Share this page:

Altmetrics

Scopus Citations

Actions (login may be required)

View Item
Report issue / request change

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340   general-enquiries@open.ac.uk