The Open UniversitySkip to content
 

Purification of anti-MUC1 antibodies by peptide mimotope affinity chromatography using peptides derived from a polyvalent phage display library

Smith, Richard G.; Missailidis, Sotiris and Price, Michael R. (2002). Purification of anti-MUC1 antibodies by peptide mimotope affinity chromatography using peptides derived from a polyvalent phage display library. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 766(1) pp. 13–26.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1016/S0378-4347(01)00422-4
Google Scholar: Look up in Google Scholar

Abstract

A polyvalent, lytic phage display system (T7Select415-lb) displaying a random peptide library has been investigated for its ability to discover novel mimotopes reactive with the therapeutic monoclonal antibody C595. Sequence analysis of enriched phage lead to the identification of a predominant sequence RNREAPRGKICS, and two other consensus sequences RXXP and RXP. The novel synthetic peptide RNREAPRGKICS was linked to beaded agarose and the performance as a mimotope affinity chromatography matrix evaluated. Antibody purified using the novel matrix was found to be of higher specific reactivity than antibody purified using the conventional epitope matrix (peptide APDTRPAPG). The RNREAPRGKICS peptide binding to C595 demonstrated a higher equilibrium association constant (K-A=0.75 x 10(6)) than the epitope peptide (K-A=0.16 x 10(6)). Circular dichroism showed that the novel peptide had a more highly ordered structure at 4 degrees C and room temperature, than the epitope peptide.

Item Type: Journal Article
ISSN: 1570-0232
Extra Information: Some of the symbols may not have transferred correctly into this bibliographic record and/or abstract.
Keywords: affinity purification; polyvalent phage display library; anti-MUC1 antibodies; peptides; C595; bladder cancer
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 3970
Depositing User: Sotiris Missailidis
Date Deposited: 05 Jul 2006
Last Modified: 10 Mar 2014 10:31
URI: http://oro.open.ac.uk/id/eprint/3970
Share this page:

Actions (login may be required)

View Item
Report issue / request change

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340   general-enquiries@open.ac.uk