The Open UniversitySkip to content
 

Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3.

Lopez-Ramirez, Miguel Alejandro; Male, David Kingsley; Wang, Chunfang; Sharrack, Basil; Wu, Dongsheng and Romero, Ignacio Andres (2013). Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3. Fluids and Barriers of the CNS, 10(1), article no. 27.

Full text available as:
[img]
Preview
PDF (Version of Record) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (300kB) | Preview
DOI (Digital Object Identifier) Link: https://doi.org/10.1186/2045-8118-10-27
Google Scholar: Look up in Google Scholar

Abstract

Background: The human cerebral microvascular endothelial cell line, hCMEC/D3, has been used extensively to model the blood–brain barrier (BBB) in vitro. Recently, we reported that cytokine-treatment induced loss of brain endothelial barrier properties. In this study, we further determined the gene expression pattern of hCMEC/D3 cells in response to activation with TNFα and IFNγ.

Findings: Using a microarray approach, we observed that expression of genes involved in the control of barrier permeability, including inter-brain endothelial junctions (e.g. claudin-5, MARVELD-2), integrin-focal adhesions complexes (e.g. integrin β1, ELMO-1) and transporter systems (e.g. ABCB1, SLC2A1), are altered by pro-inflammatory cytokines.

Conclusions: Our study shows that previously-described cytokine-induced changes in the pattern of gene expression of endothelium are reproduced in hCMEC/D3 cells, suggesting that this model is suitable to study inflammation at the BBB, while at the same time it has provided insights into novel key molecular processes that are altered in brain endothelium during neuroinflammation, such as modulation of cell-to-matrix contacts.

Item Type: Journal Item
Copyright Holders: 2013 Lopez-Ramirez et al.
ISSN: 2045-8118
Keywords: blood–brain barrier; transcriptome; cytokines; transporter systems; inter-brain endothelial junctions; integrin-focal adhesions
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Innovation, Knowledge & Development research centre (IKD)
Item ID: 39568
Depositing User: Ignacio A Romero
Date Deposited: 25 Feb 2014 14:47
Last Modified: 08 Dec 2018 05:32
URI: http://oro.open.ac.uk/id/eprint/39568
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU