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The hCMEC/D3 cell line as a model of the human blood brain barrier

Weksler, Babette; Romero, Ignacio A. and Couraud, Pierre-Olivier (2013). The hCMEC/D3 cell line as a model of the human blood brain barrier. Fluids and barriers of the CNS, 10(1), article no. 16.

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Since the first attempts in the 1970s to isolate cerebral microvessel endothelial cells (CECs) in order to model the blood-brain barrier (BBB) in vitro, the need for a human BBB model that closely mimics the in vivo phenotype and is reproducible and easy to grow, has been widely recognized by cerebrovascular researchers in both academia and industry. While primary human CECs would ideally be the model of choice, the paucity of available fresh human cerebral tissue makes wide-scale studies impractical. The brain microvascular endothelial cell line hCMEC/D3 represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). Indeed, since the development of this cell line in 2005 over 100 studies on different aspects of cerebral endothelial biology and pharmacology have been published. Here we review the suitability of this cell line as a human BBB model for pathogenic and drug transport studies and we critically consider its advantages and limitations.

Item Type: Journal Item
Copyright Holders: 2013 The Authors; licensee BioMed Central Ltd.
ISSN: 2045-8118
Keywords: blood–brain barrier; immortalized cell line; brain endothelium; in vitro model
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 39556
Depositing User: Ignacio A Romero
Date Deposited: 25 Feb 2014 12:46
Last Modified: 07 Dec 2018 10:21
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