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Martens, Wendy; Sanen, Kathleen; Georgiou, Melanie; Struys, Tom; Bronckaers, Annelies; Ameloot, Marcel; Phillips, James and Lambrichts, Ivo
(2014).
DOI: https://doi.org/10.1096/fj.13-243980
URL: http://www.fasebj.org/content/28/4/1634
Abstract
In the present study, we evaluated the differentiation potential of human dental pulp stem cells (hDPSCs) toward Schwann cells, together with their functional capacity with regard to myelination and support of neurite outgrowth in vitro. Successful Schwann cell differentiation was confirmed at the morphological and ultrastructural level by transmission electron microscopy. Furthermore, compared to undifferentiated hDPSCs, immunocytochemistry and ELISA tests revealed increased glial marker expression and neurotrophic factor secretion of differentiated hDPSCs (d-hDPSCs), which promoted survival and neurite outgrowth in 2-dimensional dorsal root ganglia cultures. In addition, neurites were myelinated by d-hDPSCs in a 3-dimensional collagen type I hydrogel neural tissue construct. This engineered construct contained aligned columns of d-hDPSCs that supported and guided neurite outgrowth. Taken together, these findings provide the first evidence that hDPSCs are able to undergo Schwann cell differentiation and support neural outgrowth in vitro, proposing them to be good candidates for cell-based therapies as treatment for peripheral nerve injury.
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About
- Item ORO ID
- 39193
- Item Type
- Journal Item
- ISSN
- 1530-6860
- Project Funding Details
-
Funded Project Name Project ID Funding Body Not Set 05G02BOF Hasselt University Not Set K200713N FWO Not Set Not Set Interreg Euregio Meuse-Rhine IV-A consortium BioMIMedics Not Set GO29112FWO FWO Not Set Not Set Boehringer Ingelheim Fonds - Keywords
- neural regeneration; nerve repair; glial cell; myelination; cellular hydrogel
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 2013 FASEB
- Depositing User
- James B. Phillips