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Human dental pulp stem cells can differentiate into Schwann cells and promote and guide neurite outgrowth in an aligned tissue-engineered collagen construct in vitro

Martens, Wendy; Sanen, Kathleen; Georgiou, Melanie; Struys, Tom; Bronckaers, Annelies; Ameloot, Marcel; Phillips, James and Lambrichts, Ivo (2014). Human dental pulp stem cells can differentiate into Schwann cells and promote and guide neurite outgrowth in an aligned tissue-engineered collagen construct in vitro. FASEB Journal, 28(4) pp. 1634–1643.

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URL: http://www.fasebj.org/content/28/4/1634
DOI (Digital Object Identifier) Link: https://doi.org/10.1096/fj.13-243980
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Abstract

In the present study, we evaluated the differentiation potential of human dental pulp stem cells (hDPSCs) toward Schwann cells, together with their functional capacity with regard to myelination and support of neurite outgrowth in vitro. Successful Schwann cell differentiation was confirmed at the morphological and ultrastructural level by transmission electron microscopy. Furthermore, compared to undifferentiated hDPSCs, immunocytochemistry and ELISA tests revealed increased glial marker expression and neurotrophic factor secretion of differentiated hDPSCs (d-hDPSCs), which promoted survival and neurite outgrowth in 2-dimensional dorsal root ganglia cultures. In addition, neurites were myelinated by d-hDPSCs in a 3-dimensional collagen type I hydrogel neural tissue construct. This engineered construct contained aligned columns of d-hDPSCs that supported and guided neurite outgrowth. Taken together, these findings provide the first evidence that hDPSCs are able to undergo Schwann cell differentiation and support neural outgrowth in vitro, proposing them to be good candidates for cell-based therapies as treatment for peripheral nerve injury.

Item Type: Journal Item
Copyright Holders: 2013 FASEB
ISSN: 1530-6860
Project Funding Details:
Funded Project NameProject IDFunding Body
Not Set05G02BOFHasselt University
Not SetK200713NFWO
Not SetNot SetInterreg Euregio Meuse-Rhine IV-A consortium BioMIMedics
Not SetGO29112FWOFWO
Not SetNot SetBoehringer Ingelheim Fonds
Keywords: neural regeneration; nerve repair; glial cell; myelination; cellular hydrogel
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 39193
Depositing User: James Phillips
Date Deposited: 23 Dec 2013 10:25
Last Modified: 04 Oct 2016 12:58
URI: http://oro.open.ac.uk/id/eprint/39193
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