Missailidis, Sotiris and Brady, Kevin
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|DOI (Digital Object Identifier) Link:||http://doi.org/10.1226/1588290921|
|Google Scholar:||Look up in Google Scholar|
This chapter presents the technical details for the study of antibody-antigen interactions using fluorescent spectroscopy. It identifies different methodologies for performing titrations depending on the availability and properties of the material, as well as providing information on ploting titration curves based on the quenching of the natural antibody fluorescence. Finally, it provides information on co-operativity and multiple antigen-antibody interactions and the way to handle those.
|Item Type:||Book Chapter|
|Keywords:||fluorescence spectroscopy; antibody-antigen interactions; data fitting; study of binding properties|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Sotiris Missailidis|
|Date Deposited:||05 Jul 2006|
|Last Modified:||23 Feb 2016 21:07|
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