Koles, Kate; McDowell, William; Mileusnic, Radmila and Rose, Steven P.R.
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The majority of plasma membrane components are glycosylated. It is now widely accepted that this post-translational modification is crucial during the establishment, maintenance and function of the nervous system. Despite its significance, structural information about the glycosylation of nervous system specific glycoproteins is very limited. In the present study the major glycan structure of the chicken synaptic plasma membrane (SPM) associated glycoprotein glycans were determined. N-glycans were released by hydozinnolysis, labelled with 2-aminobenzam,ide, treated with neuraminidase and subsequently fractionated by size exclusion chromatography. Individual fractions were characterized by combination of high-pressure liquid chromatography, exoglicosidase treatment or reagent array analysis method (RAAM). In addition to oligomannose-type glycans, core-fucosylated complex glycans with biantennary bisecting glycans carrying the LewisX epitope were most abundant. The overall chicken glycan profile was strikingly similar to the rat brain glycan profile. The presence of the LewisX determinant in relatively large proportions suggests a tissue-specific function for these glycans.
|Item Type:||Journal Article|
|Keywords:||chick brain; glycoproteins; synaptic plasma membrane|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Radmila Mileusnic|
|Date Deposited:||03 Jul 2006|
|Last Modified:||23 Feb 2016 21:32|
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