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A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury

East, Emma; Johns, Noemie; Georgiou, Melanie; Golding, Jon; Loughlin, Jane; Kingham, Paul and Phillips, James (2013). A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury. Regenerative Medicine, 8(6) pp. 739–746.

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URL: http://www.futuremedicine.com/toc/rme/8/6
DOI (Digital Object Identifier) Link: https://doi.org/10.2217/rme.13.61
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Abstract

Aim: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success. Materials & methods: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control. Results: NCSCs and differentiated Schwann cell-like adipose derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent. Conclusion: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis.

Item Type: Article
Copyright Holders: 2013 The Authors
ISSN: 1746-076X
Project Funding Details:
Funded Project NameProject IDFunding Body
Not Set080309Wellcome Trust
Not SetNot SetSwedish Research Council
Keywords: 3D cell culture; astrocytes; CNS; reactive gliosis; spinal cord; stem cell therapy
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Related URLs:
Item ID: 38830
Depositing User: James Phillips
Date Deposited: 25 Oct 2013 08:40
Last Modified: 31 Jan 2017 17:19
URI: http://oro.open.ac.uk/id/eprint/38830
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