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Targeting of Antibodies using Aptamers

Missailidis, Sotiris (2003). Targeting of Antibodies using Aptamers. In: Lo, Benny K.C. ed. Antibody engineering: methods and protocols. 2nd edition. Methods in Molecular Biology, 51. Humana Press, pp. 547–555.

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DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1226/1588290921
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Abstract

The chapter presents a methodology for the rapid selection of aptamers against antibody targets. It is a detailed account of the various methodological steps that describe the selection of aptamers, including PCR steps, buffers to be used, target immobilisation, partitioning and amplification of aptamers, clonning and sequencing, to results in high affinity and specificity ligands for the chosen target antibody.

Item Type: Book Chapter
ISBN: 1-58829-092-1, 978-1-58829-092-2
Keywords: aptamers; antibodies; aptamer selection; molecular biology techniques
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 3878
Depositing User: Sotiris Missailidis
Date Deposited: 03 Jul 2006
Last Modified: 10 Mar 2014 17:00
URI: http://oro.open.ac.uk/id/eprint/3878
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