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|DOI (Digital Object Identifier) Link:||http://doi.org/10.1226/1588290921|
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The chapter presents a methodology for the rapid selection of aptamers against antibody targets. It is a detailed account of the various methodological steps that describe the selection of aptamers, including PCR steps, buffers to be used, target immobilisation, partitioning and amplification of aptamers, clonning and sequencing, to results in high affinity and specificity ligands for the chosen target antibody.
|Item Type:||Book Chapter|
|Keywords:||aptamers; antibodies; aptamer selection; molecular biology techniques|
|Academic Unit/Department:||Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Sotiris Missailidis|
|Date Deposited:||03 Jul 2006|
|Last Modified:||02 Aug 2016 16:31|
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