The Open UniversitySkip to content

Targeting of Antibodies using Aptamers

Missailidis, Sotiris (2003). Targeting of Antibodies using Aptamers. In: Lo, Benny K.C. ed. Antibody engineering: methods and protocols. 2nd edition. Methods in Molecular Biology, 51. Humana Press, pp. 547–555.

Full text available as:
PDF (Not Set) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (71Kb)
DOI (Digital Object Identifier) Link:
Google Scholar: Look up in Google Scholar


The chapter presents a methodology for the rapid selection of aptamers against antibody targets. It is a detailed account of the various methodological steps that describe the selection of aptamers, including PCR steps, buffers to be used, target immobilisation, partitioning and amplification of aptamers, clonning and sequencing, to results in high affinity and specificity ligands for the chosen target antibody.

Item Type: Book Chapter
ISBN: 1-58829-092-1, 978-1-58829-092-2
Keywords: aptamers; antibodies; aptamer selection; molecular biology techniques
Academic Unit/Department: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 3878
Depositing User: Sotiris Missailidis
Date Deposited: 03 Jul 2006
Last Modified: 06 Oct 2016 14:00
Share this page:


Scopus Citations

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

▼ Automated document suggestions from open access sources

Actions (login may be required)

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340