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Serum deprivation and re-addition: Effects on cyclooxygenase inhibitor sensitivity in cultured glia

Phillips, James and Pearce, Brian (2005). Serum deprivation and re-addition: Effects on cyclooxygenase inhibitor sensitivity in cultured glia. Inflammopharmacology, 13(5-6) pp. 431–439.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1163/156856005774649368
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Abstract

A number of drugs were assessed for their ability to inhibit stimulus-evoked prostanoid synthesis in cultured glia. These drugs included non-selective cyclooxygenase (COX) inhibitors and those considered to be selective for the inducible isoform of this enzyme (COX-2). Experiments were carried out on normal cultures and those which had been maintained in serum-free growth medium for four days then re-exposed to serum for a further seven days. All of the drugs tested elicited concentration-dependent inhibitions of arachidonic acid (AA)-stimulated thromboxane B2 (TXB2) accumulation in normal cultures with the following rank order of potency: indomethacin > piroxicam > nimesulide = NS398 > ibuprofen ≫ aspirin > paracetamol. In cultures which had been deprived of serum for four days, basal and AA-stimulated TXB2 production was considerably reduced, as was the amount of COX immunoreactivity determined by Western blotting. Basal and AA-stimulated TXB2 production together with COX immunoreactivity were restored to control levels by the re-addition of serum to serum-deprived cultures for 7 days. In these cultures, the rank order of potency was: indomethacin > piroxicam ≫ ibuprofen > nimesulide = NS398 ≫ aspirin > paracetamol; however, there were marked charges in the apparent IC50 values for particular drugs. Indomethacin, piroxicam and aspirin were very similar to control, but the potencies of ibuprofen (3-fold), NS398 (30-fold) and nimesulide (40-fold) were found to be decreased when compared to control. Paracetamol, on the other hand, was found to be almost 3-fold more potent under these conditions. Glia appear to express a COX with a novel sensitivity to particular inhibitors following serum deprivation and re-addition.

Item Type: Journal Article
ISSN: 0925-4692
Extra Information: Some of the symbols may not have transferred correctly into this bibliographic record.
Keywords: Cultured Glia; Cyclooxygenases; Cyclooxygenase inhibitors; Serum Deprivation; Serum Readdition
Academic Unit/Department: Science
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 3859
Depositing User: James Phillips
Date Deposited: 06 Jul 2006
Last Modified: 07 Mar 2014 13:45
URI: http://oro.open.ac.uk/id/eprint/3859
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