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Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering

Phillips, James B.; King, Von R.; Ward, Zoë; Porter, Rebecca A.; Priestley, John V. and Brown, Robert A. (2004). Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering. Biomaterials, 25(14) pp. 2769–2779.

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Fibronectin (Fn) materials prepared from human plasma have been used in various forms as substrates for tissue engineering. Such purposes require that the soluble protein aggregates into insoluble fibrous structures which encourage the attachment and migration of cells. The method of aggregation due to mechanical shear was investigated by applying fluid shear forces directly to a viscous solution of Fn. Structural analysis revealed that mechanical shear resulted in the formation ofan orientated fibrous protein material that was less soluble than its non-sheared counterpart. The suitability of this shear aggregated Fn material for CNS repair purposes was assessed in vitro where it supported the growth of fibroblasts, S100 immunoreactive Schwann cells and GFAP immunoreactive astrocytes. Implantation of the shear aggregated Fn material into a rat model ofspinal cord injury provided a permissive environment for axonal growth. This was extended using an impermeable coating to improve orientation and straightness of axonal growth.

Item Type: Journal Article
Copyright Holders: 2003 Elsevier Ltd.
ISSN: 1878-5905
Keywords: fibronectin; protein aggregation; spinal cord repair; tissue engineering; Schwann cell; neural fibroblast; astrocyte
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 3851
Depositing User: James Phillips
Date Deposited: 06 Jul 2006
Last Modified: 19 Jan 2016 10:26
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