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Characterization of PAH/DPPG layer-by-layer films by VUV spectroscopy

Duarte, Andreia A.; Gomes, Paulo J.; Ribeiro, Jorge H. F.; Ribeiro, Paulo A.; Hoffmann, Søren V.; Mason, Nigel J.; Oliveira, Osvaldo N. and Raposo, Maria (2013). Characterization of PAH/DPPG layer-by-layer films by VUV spectroscopy. The European Physical Journal E, 36(9), article no. 98.

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The spectroscopic characterization of layer-by-layer (LbL) films containing liposomes is essential not only for determining the precise film architecture but also to guide the design of drug delivery systems. In this study we provide the first report of vacuum ultraviolet spectroscopy (VUV) characterization of LbL films made with liposomes from 1.2-dipalmitoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (Sodium Salt) (DPPG) alternated with poly(allylamine hydrochloride) (PAH). Measurements in the 6.0–9.5 eV range allowed us to identify the electronic transitions responsible for the spectra, which were assigned to carboxyl, hydroxyl and phosphate groups in DPPG while the PAH spectra were governed by electronic transitions in the amino groups. The surface mass density of the LbL films could be determined, from which the formation of a DPPG bilayer was inferred. This rupture of the liposomes into bilayers was confirmed with atomic force microscopy measurements. In subsidiary experiments we ensured that the UV irradiation in vacuum had negligible damage in the DPPG liposomes during the course of the VUV measurements. In addition to demonstrating the usefulness of VUV spectroscopy, the results presented here may be exploited in biological applications of liposome-containing films.

Item Type: Journal Item
Copyright Holders: 2013 EDP Sciences / Societ`a Italiana di Fisica / Springer-Verlag
ISSN: 1292-8941
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetFundaçāo para a Ciência e Tecnologia (Portugal)
Not SetNot SetFAPESP
Not SetNot SetCNPq (Brazil)
CAPES-Brazil/Grices-PortugalNot SetNot Set
Not SetSFRH/BD/62229/2009Not Set
Not SetSFRH/BD/35954/2007Not Set
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Physics
Item ID: 38493
Depositing User: Astrid Peterkin
Date Deposited: 25 Sep 2013 09:18
Last Modified: 10 Dec 2018 22:13
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