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Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes

Kashir, Junaid; Jones, Celine; Lee, Hoi Chang; Rietdorf, Katja; Nikiforaki, Dimitra; Durrans, Claire; Ruas, Margarida; Tee, Sze Tian; Heindryckx, Bjorn; Galione, Antony; De Sutter, Petra; Fissore, Rafael A.; Parrington, John and Coward, Kevin (2011). Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes. Human Reproduction, 26(12) pp. 3372–87.

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URL: http://humrep.oxfordjournals.org/content/26/12/337...
DOI (Digital Object Identifier) Link: https://doi.org/10.1093/humrep/der336
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Abstract

We demonstrate, for the first time, the production of active recombinant human PLCζ protein which retained the ability to elicit characteristic Ca(2+) oscillations in mouse oocytes, an ability which was eliminated by an infertility-linked mutation. These findings advance our understanding of PLCζ, and provide a critical step forward in obtaining purified PLCζ protein as a potential therapeutic agent for oocyte activation deficiency.

Item Type: Journal Item
Copyright Holders: 2011 The Authors
ISSN: 1460-2350
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetThe Royal Society (UK)
Keywords: oocyte activation; assisted oocyte activation; sperm; phospholipase C zeta (PLC zeta); male infertility
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 38457
Depositing User: Katja Rietdorf
Date Deposited: 19 Sep 2013 10:22
Last Modified: 09 Dec 2018 03:18
URI: http://oro.open.ac.uk/id/eprint/38457
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