Dermon, C. R.; Zikopolous, B.; Panagis, L.; Harrison, E.; Lancashire, C. L.; Mileusnic, R. and Stewart, M.G.
(2002).
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1046/j.1460-9568.2002.02177.x |
|---|---|
| Google Scholar: | Look up in Google Scholar |
Abstract
One-day-old domestic chicks were injected i.p. with bromodeoxyuridine (BrdU) before training on a one-trial passive avoidance task where the aversive experience was a bead coated with a bitter tasting substance, methyl anthranilate (MeA). Animals were tested 24 h later; those avoiding (if MeA-trained) or pecking if water (W)-trained (which they peck appetitively), along with a group of untrained naïve chicks, were used to determine cell proliferation either 24 h or 9 days post BrdU injection. In all three groups, BrdU positive cells were identified sparsely throughout the forebrain but labelling was pronounced around ventricular zone (VZ) surfaces at both 24 h and 9 days post-BrdU-injection. Double immunolabelling with neuronal specific antibodies, to either NeuN, or β-tubulin III, confirmed that most BrdU labelled cells appeared to be neurons. Unbiased stereological analysis of labelled cells in selected forebrain areas 24 h post BrdU injection showed a significant MeA-training induced increase in labelled cells in both the dorsal VZ surface bordering the intermediate and medial hyperstriatum ventrale (IMHV) and the tuberculum olfactorium (TO). By 9 days post-BrdU-injection, there was a significantly greater number of BrdU labelled cells in MeA-trained birds within the IMHV, lobus parolfactorius (LPO) and TO. These results demonstrate that avoidance training in 1-day-old chicks has a marked effect on cell proliferation, in the LPO and IMHV, regions of the chick previously identified as a key loci of memory formation, and in a second region (TO), which has olfactory functions, but has not been previously investigated in relation to avoidance learning.
| Item Type: | Article |
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| ISSN: | 0953-816X |
| Keywords: | neurogenesis; memory; chick |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Interdisciplinary Research Centre: | Biomedical Research Network (BRN) |
| Item ID: | 3778 |
| Depositing User: | Radmila Mileusnic |
| Date Deposited: | 30 Jun 2006 |
| Last Modified: | 04 Oct 2016 09:50 |
| URI: | http://oro.open.ac.uk/id/eprint/3778 |
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