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Converting fluorescence data into Ca2+ concentration

Bootman, Martin D.; Rietdorf, Katja; Collins, Tony; Walker, Simon and Sanderson, Michael (2013). Converting fluorescence data into Ca2+ concentration. Cold Spring Harbor Protocols, 2013(2) pp. 126–129.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1101/pdb.prot072827
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Abstract

In many situations, fluorescent Ca2+ reporters are used to simply indicate that a change of Ca2+ concentration has occurred. Monitoring the emission from a Ca2+-sensitive indicator can be sufficient to tell whether a signal has arisen, and what its kinetic/spatial parameters were. The emission from an indicator does not have a linear relationship to the Ca2+ concentration within a cell; rather, the relationship between fluorescence emission and Ca2+ concentration is described by a logistic function. Simply recording fluorescence emission, therefore, provides a relative indication of the magnitude of a Ca2+ signal that should not be used for generating mean amplitude data. However, with a little consideration and effort, the fluorescence output can be calibrated to yield actual Ca2+ concentration.

Item Type: Journal Item
Copyright Holders: 2013 Cold Spring Harbor Laboratory Press
ISSN: 1559-6095
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 36741
Depositing User: Martin Bootman
Date Deposited: 28 Feb 2013 14:32
Last Modified: 08 Dec 2018 09:53
URI: http://oro.open.ac.uk/id/eprint/36741
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