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High-density lipoproteins limit neutrophil-induced damage to the blood–brain barrier in vitro

Bao Dang, Quoc; Lapergue, Bertrand; Tran-Dinh, Alexy; Diallo, Devy; Moreno, Juan-Antonio; Mazighi, Mikael; Romero, Ignacio A.; Weksler, Babette; Michel, Jean-Baptiste; Amarenco, Pierre and Meilhac, Olivier (2013). High-density lipoproteins limit neutrophil-induced damage to the blood–brain barrier in vitro. Journal of Cerebral Blood Flow & Metabolism, 33(4) pp. 575–582.

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Breakdown of the blood–brain barrier (BBB) is a key step associated with ischemic stroke and its increased permeability causes extravasation of plasma proteins and circulating leukocytes. Polymorphonuclear neutrophil (PMN) proteases may participate in BBB breakdown. We investigated the role of PMNs in ischemic conditions by testing their effects on a model of BBB in vitro, under oxygen-glucose deprivation (OGD) to mimic ischemia, supplemented or not with high-density lipoproteins (HDLs) to assess their potential protective effects. Human cerebral endothelial cells cultured on transwells were incubated for 4 hours under OGD conditions with or without PMNs and supplemented or not with HDLs or alpha-1 antitrypsin (AAT, an elastase inhibitor). The integrity of the BBB was then assessed and the effect of HDLs on PMN-induced proteolysis of extracellular matrix proteins was evaluated. The release of myeloperoxidase and matrix metalloproteinase 9 (MMP-9) by PMNs was quantified. Polymorphonuclear neutrophils significantly increased BBB permeability under OGD conditions via proteolysis of extracellular matrix proteins. This was associated with PMN degranulation. Addition of HDLs or AAT limited the proteolysis and associated increased permeability by inhibiting PMN activation. Our results suggest a deleterious, elastase-mediated role of activated PMNs under OGD conditions leading to BBB disruption that could be inhibited by HDLs.

Item Type: Journal Item
Copyright Holders: 2013 Nature
ISSN: 1559-7016
Keywords: blood–brain barrier; high-density lipoproteins; ischemia; neutrophil elastase; stroke
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 36406
Depositing User: Ignacio A Romero
Date Deposited: 30 Jan 2013 12:56
Last Modified: 07 Dec 2018 10:13
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