Dolatshad,, Nazanin F.; Silva, Ayona T. and Saffrey, M. Jill
Identification of GFR alpha-2 isoforms in myenteric plexus of postnatal and adult rat intestine.
Molecular Brain Research, 107(1),
Glial cell line-derived neurotrophic factor family receptor a-2 (GFR a-2) is a GPI-linked receptor that preferentially binds neurturin (NTN), a member of the glial cell line-derived neurotrophic factor (GDNF) family. Three splice isoforms of GFR a-2 have been
identified previously in mouse tissues, but the occurrence of splice isoforms in rats has not been described. The aim of this study was therefore to identify GFR a-2 splice isoforms in rat tissues using reverse transcription–polymerase chain reaction (RT–PCR) and gene
cloning. Three isoforms were identified and sequenced, and named GFR a-2(a), (b) and (c), according to the nomenclature used for the previously identified mouse isoforms. The GFR a-2(a) and (b) isoforms were identical to those previously described in mice. The GFR
a-2(c) isoform was novel. Sequences for GFR a-2(b) and (c) were deposited in the GenBank database (accession numbers GI: 16797788and 16797786, respectively). All three isoforms were expressed in the brain, kidney, and intestine of both postnatal and adult rats.
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