The Open UniversitySkip to content

Using calcium imaging as a readout of GPCR activation

Bootman, Martin and Roderick, H. Llewelyn (2011). Using calcium imaging as a readout of GPCR activation. In: Willars, Gary B and Challiss, R A John eds. Receptor Signal Transduction Protocols, Volume 746. Springer, pp. 277–296.


This is the latest version of this eprint.

Full text available as:
Full text not publicly available (Version of Record)
Due to publisher licensing restrictions, this file is not available for public download
Click here to request a copy from the OU Author.
DOI (Digital Object Identifier) Link:
Google Scholar: Look up in Google Scholar


Monitoring cellular calcium concentration using fluorescent reporters can provide a rapid, proportional assay of G-protein-coupled receptor activation. Recording calcium changes in single cells, or cell populations, is relatively straightforward, but requires careful deliberation regarding the appropriate calcium reporter and experimental approach. Here, we describe strategies to ensure that calcium changes are recorded with good fidelity and minimal invasiveness. We highlight a range of issues that need to be considered within the design of an experiment to measure cellular calcium, and suggest strategies to avoid common pit-falls.

Item Type: Book Section
Copyright Holders: 2011 Springer
ISSN: 1940-6029
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 35109
Depositing User: Martin Bootman
Date Deposited: 20 Nov 2012 11:40
Last Modified: 02 May 2018 13:46
Share this page:

Available Versions of this Item


Altmetrics from Altmetric

Citations from Dimensions

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU