Bootman, Martin and Roderick, H. Llewelyn
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|DOI (Digital Object Identifier) Link:||http://dx.doi.org/10.1007/978-1-61779-126-0_15|
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Monitoring cellular calcium concentration using fluorescent reporters can provide a rapid, proportional assay of G-protein-coupled receptor activation. Recording calcium changes in single cells, or cell populations, is relatively straightforward, but requires careful deliberation regarding the appropriate calcium reporter and experimental approach. Here, we describe strategies to ensure that calcium changes are recorded with good fidelity and minimal invasiveness. We highlight a range of issues that need to be considered within the design of an experiment to measure cellular calcium, and suggest strategies to avoid common pit-falls.
|Item Type:||Book Chapter|
|Copyright Holders:||2011 Springer|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences|
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Martin Bootman|
|Date Deposited:||20 Nov 2012 11:40|
|Last Modified:||26 Mar 2014 06:20|
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Available Versions of this Item
Using calcium imaging as a readout of GPCR activation. (deposited 25 Oct 2012 09:25)
- Using calcium imaging as a readout of GPCR activation. (deposited 20 Nov 2012 11:40) [Currently Displayed]
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