Normal Ca2+ signalling in glutathione-depleted and dithiothreitol-treated HeLa cells

Missiaen, Ludwig; Parys, Jan B.; Bootman, Martin D.; De Smedt, Humbert; Droogmans, Guy and Casteels, Rik (1993). Normal Ca2+ signalling in glutathione-depleted and dithiothreitol-treated HeLa cells. Pflügers Archiv, 423(5-6) pp. 480–484.

DOI: https://doi.org/10.1007/BF00374944

Abstract

We have investigated whether reducing agents and substances that interfere with glutathione metabolism would affect the histamine-induced rises in internal Ca2+ concentration ([Ca2+]i) in indo-1-loaded HeLa cells. Individual cells responded to 1 microM histamine with either baseline or sinusoidal Ca2+ oscillations, a single Ca2+ peak or a maintained elevation of the [Ca2+]i. Only a few cells did not respond. The sulphydryl reducing agent dithiothreitol (5 mM) did not affect these responses to histamine. A 24-h preincubation with 1 mM DL-buthionine (SR)-sulphoximine, which reduces the cellular glutathione content to less than 20% of its control value, affected neither these histamine responses, nor the [Ca2+]i rises after application of 2 μM thapsigargin. We conclude that oxidation of critical sulphydryl groups is not required for the normal response to histamine and also that glutathione plays no role in agonist-induced Ca2+ signalling in HeLa cells.

Viewing alternatives

Metrics

Public Attention

Altmetrics from Altmetric

Number of Citations

Citations from Dimensions
No digital document available to download for this item

Item Actions

Export

About

Recommendations