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Missiaen, Ludwig; Parys, Jan B.; Bootman, Martin D.; De Smedt, Humbert; Droogmans, Guy and Casteels, Rik
(1993).
DOI: https://doi.org/10.1007/BF00374944
Abstract
We have investigated whether reducing agents and substances that interfere with glutathione metabolism would affect the histamine-induced rises in internal Ca2+ concentration ([Ca2+]i) in indo-1-loaded HeLa cells. Individual cells responded to 1 microM histamine with either baseline or sinusoidal Ca2+ oscillations, a single Ca2+ peak or a maintained elevation of the [Ca2+]i. Only a few cells did not respond. The sulphydryl reducing agent dithiothreitol (5 mM) did not affect these responses to histamine. A 24-h preincubation with 1 mM DL-buthionine (SR)-sulphoximine, which reduces the cellular glutathione content to less than 20% of its control value, affected neither these histamine responses, nor the [Ca2+]i rises after application of 2 μM thapsigargin. We conclude that oxidation of critical sulphydryl groups is not required for the normal response to histamine and also that glutathione plays no role in agonist-induced Ca2+ signalling in HeLa cells.
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About
- Item ORO ID
- 35063
- Item Type
- Journal Item
- ISSN
- 1432-2013
- Keywords
- cytosolic Ca2+ signal; thiol reagent; HeLa cells
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 1993 Springer-Verlag(?)
- Depositing User
- Martin Bootman