Mechanisms underlying the neuronal calcium sensor-1-evoked enhancement of exocytosis in PC12 Cells

Koizumi, Schuichi; Rosa, Patrizia; Willars, Gary B.; Challiss, R. A. John; Taverna, Elena; Francolini, Maura; Bootman, Martin D; Inoue, Kazuhide; Roder, John and Jeromin, Andreas (2002). Mechanisms underlying the neuronal calcium sensor-1-evoked enhancement of exocytosis in PC12 Cells. Journal of Biological Chemistry, 277(33) pp. 30315–30324.

DOI: https://doi.org/10.1074/jbc.M201132200

Abstract

Neuronal calcium sensor-1 (NCS-1) or the originally identified homologue frequenin belongs to a superfamily of EF-hand calcium binding proteins. Although NCS-1 is thought to enhance synaptic efficacy or exocytosis mainly by activating ion channel function, the detailed molecular basis for the enhancement is still a matter of debate. Here, mechanisms underlying the NCS-1-evoked enhancement of exocytosis were investigated using PC12 cells overexpressing NCS-1. NCS-1 was found to have a broad distribution in the cells being partially distributed in the cytosol and associated to vesicles and tubular-like structures. Biochemical and immunohistochemical studies indicated that NCS-1 partially colocalized with the light synaptic vesicle marker synaptophysin. When stimulated with UTP or bradykinin, agonists to phospholipase C-linked receptors, NCS-1 enhanced the agonist-mediated elementary and global Ca2+ signaling and increased the levels of downstream signals of phosphatidylinositol 4-kinase. NCS-1 enhanced the UTP-evoked exocytosis but not the depolarization-evoked Ca2+responses or exocytosis, suggesting that the enhancement by NCS-1 should involve phospholipase C-linked receptor-mediated signals rather than the Ca2+ channels or exocytotic machinery per se. Taken together, NCS-1 enhances phosphoinositide turnover, resulting in enhancement of Ca2+signaling and exocytosis. This is a novel regulatory mechanism of exocytosis that might involve the activation of phosphatidylinositol 4-kinase.

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