Copy the page URI to the clipboard
Peppiatt, Claire M.; Collins, Tony J.; Mackenzie, Lauren; Conway, Stuart J.; Holmes, Andrew B.; Bootman, Martin; Berridge, Michael J.; Seo, Jeong T. and Roderick, H. Llewelyn
(2003).
DOI: https://doi.org/10.1016/S0143-4160(03)00026-5
Abstract
The action of 2-aminoethoxydiphenyl borate (2-APB) on Ca2+ signalling in HeLa cells and cardiac myocytes was investigated. Consistent with other studies, we found that superfusion of cells with 2-APB rapidly inhibited inositol 1,4,5-trisphosphate (InsP3)-mediated Ca2+ release and store-operated Ca2+ entry (SOC). In addition to abrogating hormone-evoked Ca2+ responses, 2-APB could antagonise Ca2+ signals evoked by a membrane permeant InsP3 ester. 2-APB also slowed the recovery of intracellular Ca2+ signals consistent with an effect on Ca2+ ATPases. The inhibitory action of 2-APB on InsP3 receptors (InsP3Rs), SOC channels and Ca2+ pumps persisted for several minutes after washout of the compound. Application of 2-APB to unstimulated cells had no effect on subsequent Ca2+responses suggesting that it has a use-dependent action. Mitochondria in cells treated with 2-APB showed a rapid and slowly reversible swelling. 2-APB did not cause the mitochondria to depolarise, but it reduced the extent of mitochondrial calcium uptake. Although 2-APB has been demonstrated not to affect voltage-operated Ca2+ channels or ryanodine receptors, we found that it gave a concentration-dependent long-lasting inhibition of Ca2+ signalling in electrically-stimulated cardiac myocytes, where InsP3Rs and SOC channels do not play a significant role. Our data suggest that 2-APB has multiple cellular targets, a use-dependent action, is difficult to reverse and may affect Ca2+ signalling in cell types where InsP3 and SOC are not active.
Viewing alternatives
Metrics
Public Attention
Altmetrics from AltmetricNumber of Citations
Citations from Dimensions-
Request a copy from the authorVersion of Record (PDF)
This file is not available for public download
Item Actions
Export
About
- Item ORO ID
- 34961
- Item Type
- Journal Item
- ISSN
- 0143-4160
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 2003 Elsevier Science Ltd
- Depositing User
- Martin Bootman