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The spatial pattern of atrial cardiomyocyte calcium signalling modulates contraction

Mackenzie, Lauren; Roderick, H. Llewelyn; Berridge, Michael J.; Conway, Stuart J. and Bootman, Martin D. (2004). The spatial pattern of atrial cardiomyocyte calcium signalling modulates contraction. Journal of Cell Science, 117(26) pp. 6327–6337.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1242/jcs.01559
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Abstract

We examined the regulation of calcium signalling in atrial cardiomyocytes during excitation-contraction coupling, and how changes in the distribution of calcium impacts on contractility. Under control conditions, calcium transients originated in subsarcolemmal locations and showed local regeneration through activation of calcium-induced calcium release from ryanodine receptors. Despite functional ryanodine receptors being expressed at regular (~2 μm) intervals throughout atrial myocytes, the subsarcolemmal calcium signal did not spread in a fully regenerative manner through the interior of a cell. Rather, there was a diminishing centripetal propagation of calcium. The lack of regeneration was due to mitochondria and SERCA pumps preventing the inward movement of calcium. Inhibiting these calcium buffering mechanisms allowed the globalisation of action potential-evoked responses. In addition, physiological positive inotropic agents, such as endothelin-1 and β-adrenergic agonists, as well as enhanced calcium current, calcium store loading and inositol 1,4,5-trisphosphate infusion also led to regenerative global responses. The consequence of globalising calcium signals was a significant increase in cellular contraction. These data indicate how calcium signals and their consequences are determined by the interplay of multiple subcellular calcium management systems.

Item Type: Journal Item
Copyright Holders: 2004 The Company of Biologists Limited
ISSN: 1477-9137
Keywords: calcium; contraction; myocyte
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
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Item ID: 34955
Depositing User: Martin Bootman
Date Deposited: 02 May 2013 10:49
Last Modified: 07 Dec 2018 18:31
URI: http://oro.open.ac.uk/id/eprint/34955
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