Higazi, Daniel R.; Fearnley, Claire J.; Drawnel, Faye M.; Talasila, Amarnath; Corps, Elaine M.; Ritter, Oliver; McDonald, Fraser; Mikoshiba, Katsuhiko; Bootman, Martin D. and Roderick, H. Llewelyn
(2009).
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| DOI (Digital Object Identifier) Link: | http://dx.doi.org/doi:10.1016/j.molcel.2009.02.005 |
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| Google Scholar: | Look up in Google Scholar |
Abstract
Ca(2+) elevations are fundamental to cardiac physiology-stimulating contraction and regulating the gene transcription that underlies hypertrophy. How Ca(2+) specifically controls gene transcription on the background of the rhythmic Ca(2+) increases required for contraction is not fully understood. Here we identify a hypertrophy-signaling module in cardiac myocytes that explains how Ca(2+) discretely regulates myocyte hypertrophy and contraction. We show that endothelin-1 (ET-1) stimulates InsP(3)-induced Ca(2+) release (IICR) from perinuclear InsP(3)Rs, causing an elevation in nuclear Ca(2+). Significantly, we show that IICR, but not global Ca(2+) elevations associated with myocyte contraction, couple to the calcineurin (CnA)/NFAT pathway to induce hypertrophy. Moreover, we found that activation of the CnA/NFAT pathway and hypertrophy by isoproterenol and BayK8644, which enhance global Ca(2+) fluxes, was also dependent on IICR and nuclear Ca(2+) elevations. The activation of IICR by these activity-enhancing mediators was explained by their ability to stimulate secretion of autocrine/paracrine ET-1.
| Item Type: | Journal Article |
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| Copyright Holders: | 2009 Cell Press |
| ISSN: | 1097-4164 |
| Academic Unit/Department: | Science > Life, Health and Chemical Sciences |
| Item ID: | 34863 |
| Depositing User: | Martin Bootman |
| Date Deposited: | 20 Nov 2012 10:30 |
| Last Modified: | 24 May 2013 07:06 |
| URI: | http://oro.open.ac.uk/id/eprint/34863 |
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