Georgiou, Melanie; Bunting, Stephen; Golding, Jon; Loughlin, Jane and Phillips, James
(2012).
| URL: | http://www.wc2012-vienna.org/index.php?id=35 |
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| Google Scholar: | Look up in Google Scholar |
Abstract
Introduction: A peripheral nerve repair device with the ability to enhance regeneration would be a promising alternative to nerve autograft repair. The growth of axons across a lesion is most effective when supported by columns of aligned Schwann cells that provide cell-level guidance, as found in an autograft. Here we report the development and testing of engineered neural tissue (ENT): aligned Schwann cells in a 3D collagen environment, which supports and guides neuronal growth.
Methods: Collagen gels containing F7 Schwann cells were tethered for 24 h to permit cellular self-alignment and then stabilised by rapid removal of interstitial fluid. This process generates sheets of ENT, which are stable tissue-like gels with cells organised within a 3D matrix. Cell alignment was monitored before and after stabilisation. Dissociated dorsal root ganglia neurons were cultured on the surface of the material for 3 days and neurite growth assessed. Sheets of ENT were rolled into columns and packed together to form the core of a repair device. Various column formats were tested in a rat sciatic nerve model.
Results: Chains of aligned Schwann cells formed within the collagen matrix and persisted following stabilisation to form a robust, aligned cellular biomaterial that promoted and guided neuronal growth in vitro and in vivo.
Conclusion: Engineered neural tissue containing aligned Schwann cells can form the basis of a functional conduit for peripheral nerve repair.
| Item Type: | Conference Item |
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| Copyright Holders: | 2012 The Authors |
| Extra Information: | Journal of Tissue Engineering and Regenerative Medicine
J Tissue Eng Regen Med 2012; 6 (Suppl. 1): 1–429. DOI: 10.1002/term.1586 pp.81-82 |
| Academic Unit/Department: | Science > Life, Health and Chemical Sciences |
| Related URLs: | |
| Item ID: | 34332 |
| Depositing User: | James Phillips |
| Date Deposited: | 13 Sep 2012 10:05 |
| Last Modified: | 14 Nov 2012 12:15 |
| URI: | http://oro.open.ac.uk/id/eprint/34332 |
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