Georgiou, Melanie; Bunting, Stephen; Golding, Jon; Loughlin, Jane and Phillips, James
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Introduction: A peripheral nerve repair device with the ability to enhance regeneration would be a promising alternative to nerve autograft repair. The growth of axons across a lesion is most effective when supported by columns of aligned Schwann cells that provide cell-level guidance, as found in an autograft. Here we report the development and testing of engineered neural tissue (ENT): aligned Schwann cells in a 3D collagen environment, which supports and guides neuronal growth.
Methods: Collagen gels containing F7 Schwann cells were tethered for 24 h to permit cellular self-alignment and then stabilised by rapid removal of interstitial fluid. This process generates sheets of ENT, which are stable tissue-like gels with cells organised within a 3D matrix. Cell alignment was monitored before and after stabilisation. Dissociated dorsal root ganglia neurons were cultured on the surface of the material for 3 days and neurite growth assessed. Sheets of ENT were rolled into columns and packed together to form the core of a repair device. Various column formats were tested in a rat sciatic nerve model.
Results: Chains of aligned Schwann cells formed within the collagen matrix and persisted following stabilisation to form a robust, aligned cellular biomaterial that promoted and guided neuronal growth in vitro and in vivo.
Conclusion: Engineered neural tissue containing aligned Schwann cells can form the basis of a functional conduit for peripheral nerve repair.
|Item Type:||Conference Item|
|Copyright Holders:||2012 The Authors|
|Extra Information:||Journal of Tissue Engineering and Regenerative Medicine
J Tissue Eng Regen Med 2012; 6 (Suppl. 1): 1–429.
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences|
|Depositing User:||James Phillips|
|Date Deposited:||13 Sep 2012 10:05|
|Last Modified:||14 Nov 2012 12:15|
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