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Expression and localization of claudins-3 and -12 in transformed human brain endothelium

Schrade, Anja; Sade, Hadassah; Couraud, Pierre-Olivier; Romero, Ignacio A; Weksler, Babette B and Niewoehner, Jens (2012). Expression and localization of claudins-3 and -12 in transformed human brain endothelium. Fluids and barriers of the CNS, 9 p. 6.

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Background: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12.

Findings: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture.

Conclusions: These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.

Item Type: Journal Article
Copyright Holders: 2012 Schrade et al
ISSN: 2045-8118
Extra Information: 5 pp.
Keywords: blood brain barrier (BBB); hCMEC/D3; claudins; tight junction; statins
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 34224
Depositing User: Ignacio A Romero
Date Deposited: 22 Aug 2012 09:47
Last Modified: 18 Jan 2016 15:38
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