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Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease

Jia, Wenjing; Whitehead, Rebekah N.; Griffiths, Lesley; Dawson, Claire; Bai, Hao; Waring, Rosemary H.; Ramsden, David B.; Hunter, John O.; Cauchi, Michael; Bessant, Conrad; Fowler, Dawn P.; Walton, Christopher; Turner, Claire and Cole, Jeffrey A. (2012). Diversity and distribution of sulphate-reducing bacteria in human faeces from healthy subjects and patients with inflammatory bowel disease. FEMS Immunology and Medical Microbiology, 65(1) pp. 55–68.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1111/j.1574-695X.2012.00935.x
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Abstract

The relative abundance of different groups of sulphate-reducing bacteria (SRB) in faecal DNA collected before and after therapy from patients suffering from Crohn's disease (CD), irritable bowel syndrome (IBS) or ulcerative colitis (UC) has been compared with that from healthy controls. Growth tests revealed that SRB were not more abundant in samples from patients with CD before treatment than in the healthy control group. For most of the 128 samples available, these preliminary results were confirmed using degenerate PCR primers that amplify the dsrAB gene. However, some samples from patients with CD before treatment contained a growth inhibitor that was absent from IBS or UC samples. In-depth sequencing of PCR-generated dsrB fragments revealed that the diversity detected was surprisingly low, with only eight strains of SRB and the sulphite-reducing bacterium, Bilophila wadsworthia, detected above the 0.1% threshold. The proportion of the two major species detected, B. wadsworthia and Desulfovibrio piger, was as high as 93.5% of the total SRB population in the healthy control group and lower in all patient groups. Four previously undescribed species were found: it is impossible to predict whether they are sulphate or sulphite-reducing bacteria.

Item Type: Journal Article
Copyright Holders: 2012 Federation of European Microbiological Societies
ISSN: 1574-695X
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetWellcome Trust
Keywords: faecal sulphate-reducing bacteria; diversity; 454 sequencing; irritable bowel syndrome; Crohn’s disease; ulcerative colitis
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Centre for Earth, Planetary, Space and Astronomical Research (CEPSAR)
Biomedical Research Network (BRN)
Item ID: 33738
Depositing User: Claire Turner
Date Deposited: 30 May 2012 15:55
Last Modified: 07 Mar 2014 13:32
URI: http://oro.open.ac.uk/id/eprint/33738
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