Jia, Wenjing; Whitehead, Rebekah N.; Griffiths, Lesley; Dawson, Claire; Bai, Hao; Waring, Rosemary H.; Ramsden, David B.; Hunter, John O.; Cauchi, Michael; Bessant, Conrad; Fowler, Dawn P.; Walton, Christopher; Turner, Claire and Cole, Jeffrey A.
|DOI (Digital Object Identifier) Link:||http://doi.org/10.1111/j.1574-695X.2012.00935.x|
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The relative abundance of different groups of sulphate-reducing bacteria (SRB) in faecal DNA collected before and after therapy from patients suffering from Crohn's disease (CD), irritable bowel syndrome (IBS) or ulcerative colitis (UC) has been compared with that from healthy controls. Growth tests revealed that SRB were not more abundant in samples from patients with CD before treatment than in the healthy control group. For most of the 128 samples available, these preliminary results were confirmed using degenerate PCR primers that amplify the dsrAB gene. However, some samples from patients with CD before treatment contained a growth inhibitor that was absent from IBS or UC samples. In-depth sequencing of PCR-generated dsrB fragments revealed that the diversity detected was surprisingly low, with only eight strains of SRB and the sulphite-reducing bacterium, Bilophila wadsworthia, detected above the 0.1% threshold. The proportion of the two major species detected, B. wadsworthia and Desulfovibrio piger, was as high as 93.5% of the total SRB population in the healthy control group and lower in all patient groups. Four previously undescribed species were found: it is impossible to predict whether they are sulphate or sulphite-reducing bacteria.
|Item Type:||Journal Article|
|Copyright Holders:||2012 Federation of European Microbiological Societies|
|Project Funding Details:||
|Keywords:||faecal sulphate-reducing bacteria; diversity; 454 sequencing; irritable bowel syndrome; Crohn’s disease; ulcerative colitis|
|Academic Unit/Department:||Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
|Interdisciplinary Research Centre:||Centre for Earth, Planetary, Space and Astronomical Research (CEPSAR)
Biomedical Research Network (BRN)
|Depositing User:||Claire Turner|
|Date Deposited:||30 May 2012 15:55|
|Last Modified:||04 Oct 2016 11:18|
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