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Differing effects of NT-3 and GDNF on dissociated enteric ganglion cells exposed to hydrogen peroxide in vitro

Korsak, Kris; Silva, Ayona T. and Saffrey, M. Jill (2012). Differing effects of NT-3 and GDNF on dissociated enteric ganglion cells exposed to hydrogen peroxide in vitro. Neuroscience Letters, 517(2) pp. 102–106.

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Oxidative stress is widely recognized to contribute to neuronal death during various pathological conditions and aging. In the enteric nervous system (ENS), reactive oxygen species have been implicated in the mechanism of age-associated neuronal loss. The neurotrophic factors neurotrophin 3 (NT-3) and glial cell line-derived neurotrophic factor (GDNF) are important in the development of enteric neurons and continue to be expressed in the gut throughout life. It has therefore been suggested that they may have a neuoprotective role in the ENS. We investigated the potential of NT-3 and GDNF to prevent death of enteric ganglion cells in dissociated cell culture after exposure to hydrogen peroxide (H2O2). H2O2 treatment resulted in a dose-dependent death of enteric neurons and glial cells, as demonstrated by MTS assay, Bis benzimide and propidium iodide staining and immunolabelling. Cultures treated with NT-3 prior to exposure showed reduced cell death compared to untreated control or GDNF-treated cultures. GDNF treatment did not affect neuronal survival in H2O2-treated cultures. These results suggest that NT-3 is able to enhance the survival of enteric ganglion cells exposed to oxidative stress.

Item Type: Journal Item
Copyright Holders: 2012 Elsevier Ireland Ltd.
ISSN: 1872-7972
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetBBSRC (Biotechnology and Biological Sciences Research Council)
Not SetNot SetOU
Keywords: neurotrophin-3; glial cell line derived neurotrophic factor; enteric ganglia; oxidative stress; hydrogen peroxide
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Related URLs:
Item ID: 33546
Depositing User: Jill Saffrey
Date Deposited: 26 Apr 2012 08:34
Last Modified: 08 Dec 2018 13:58
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