Ojo, Bunmi; Rezaie, Payam; Gabbott, Paul L.; Cowely, Thelma R.; Medvedev, Nikolay I.; Lynch, Marina A. and Stewart, Michael G.
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|DOI (Digital Object Identifier) Link:||http://dx.doi.org/doi:10.1016/j.expneurol.2011.09.025|
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Neuroglial activation is a typical hallmark of ageing within the hippocampus, and correlates with age-related cognitive deficits. We have used quantitative immunohistochemistry and morphometric analyses to investigate whether systemic treatment with the Neural Cell Adhesion Molecule (NCAM)-derived peptide FG Loop (FGL) specifically alters neuroglial activation and population densities within the aged rat hippocampus (22 months of age). A series of 50μm paraformaldehyde/acrolein-fixed sections taken throughout the dorsal hippocampus (5 animals per group) were immunostained to detect astrocytes (GFAP and S100ß) and microglial cells (CD11b/OX42 and MHCII/OX6), and analysed using computerised image analysis and optical segmentation (Image-Pro Plus, Media Cybernetics). FGL treatment reduced the density of CD11b+ and MHCII+ microglia in aged animals, concomitant with a reduction in immunoreactivity for these phenotypic markers. FGL treatment also markedly reduced GFAP immunoreactivity within all hippocampal subfields in aged animals, without exerting an appreciable effect on the density of S100ß+ cells. These results demonstrate that FGL can indeed regulate neuroglial activation and reduce microglial cell density in the aged hippocampus, and support its potential use as a therapeutic agent in age-related brain disorders.
|Item Type:||Journal Article|
|Copyright Holders:||2011 Elsevier Inc.|
|Funders:||European Union FPVI “Promemoria” programme grant (Contract no. 512012)|
|Keywords:||ageing; microglia; astrocytes; hippocampus; NCAM-derived peptide|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences|
|Depositing User:||Michael Stewart|
|Date Deposited:||27 Oct 2011 15:38|
|Last Modified:||26 Nov 2012 19:01|
Available Versions of this Item
A neural cell adhesion molecule-derived peptide, FGL, attenuates glial cell activation in the aged hippocampus. (deposited 10 Oct 2011 08:56)
- A neural cell adhesion molecule-derived peptide, FGL, attenuates glial cell activation in the aged hippocampus. (deposited 27 Oct 2011 15:38) [Currently Displayed]
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