The Open UniversitySkip to content
 

A neural cell adhesion molecule-derived peptide, FGL, attenuates glial cell activation in the aged hippocampus

Ojo, Bunmi; Rezaie, Payam; Gabbott, Paul L.; Cowely, Thelma R.; Medvedev, Nikolay I.; Lynch, Marina A. and Stewart, Michael G. (2011). A neural cell adhesion molecule-derived peptide, FGL, attenuates glial cell activation in the aged hippocampus. Experimental Neurology, 232(2) pp. 318–328.

Warning

This is the latest version of this eprint.

Full text available as:
Full text not publicly available
Due to copyright restrictions, this file is not available for public download
Click here to request a copy from the OU Author.
DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1016/j.expneurol.2011.09.025
Google Scholar: Look up in Google Scholar

Abstract

Neuroglial activation is a typical hallmark of ageing within the hippocampus, and correlates with age-related cognitive deficits. We have used quantitative immunohistochemistry and morphometric analyses to investigate whether systemic treatment with the Neural Cell Adhesion Molecule (NCAM)-derived peptide FG Loop (FGL) specifically alters neuroglial activation and population densities within the aged rat hippocampus (22 months of age). A series of 50μm paraformaldehyde/acrolein-fixed sections taken throughout the dorsal hippocampus (5 animals per group) were immunostained to detect astrocytes (GFAP and S100ß) and microglial cells (CD11b/OX42 and MHCII/OX6), and analysed using computerised image analysis and optical segmentation (Image-Pro Plus, Media Cybernetics). FGL treatment reduced the density of CD11b+ and MHCII+ microglia in aged animals, concomitant with a reduction in immunoreactivity for these phenotypic markers. FGL treatment also markedly reduced GFAP immunoreactivity within all hippocampal subfields in aged animals, without exerting an appreciable effect on the density of S100ß+ cells. These results demonstrate that FGL can indeed regulate neuroglial activation and reduce microglial cell density in the aged hippocampus, and support its potential use as a therapeutic agent in age-related brain disorders.

Item Type: Journal Article
Copyright Holders: 2011 Elsevier Inc.
ISSN: 1090-2430
Project Funding Details:
Funded Project NameProject IDFunding Body
FPVI “Promemoria” programme Contract no. 512012European Union grant (Contract no. 512012)
Keywords: ageing; microglia; astrocytes; hippocampus; NCAM-derived peptide
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 29890
Depositing User: Michael Stewart
Date Deposited: 27 Oct 2011 15:38
Last Modified: 07 Mar 2014 23:07
URI: http://oro.open.ac.uk/id/eprint/29890
Share this page:

Available Versions of this Item

Altmetrics

Scopus Citations

Actions (login may be required)

View Item
Report issue / request change

Policies | Disclaimer

© The Open University   + 44 (0)870 333 4340   general-enquiries@open.ac.uk