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Human brain endothelial cells are responsive to adenosine receptor activation

Mills, Jeffrey H.; Alabanza, Leah; Weksler, Babette B.; Couraud, Pierre-Olivier; Romero, Ignacio A. and Bynoe, Margaret S. (2011). Human brain endothelial cells are responsive to adenosine receptor activation. Purinergic Signalling, 7(2) pp. 265–273.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1007/s11302-011-9222-2
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Abstract

The blood-brain barrier (BBB) of the central nervous system (CNS) consists of a unique subset of endothelial cells that possess tight junctions which form a relatively impervious physical barrier to a large variety of blood components. Until recently, there have been no good in vitro models for studying the human BBB without the co-culture of feeder cells. The hCMEC/D3 cell line is the first stable, well-differentiated human brain endothelial cell line that grows independently in culture with characteristics that closely resemble those of resident human brain endothelial cells. As our previously published findings demonstrated the importance of adenosine receptor (AR) signaling for lymphocyte entry into the CNS, we wanted to determine if human brain endothelial cells possess the capacity to generate and respond to extracellular adenosine. Utilizing the hCMEC/D3 cell line, we determined that these cells express CD73, the cell surface enzyme that converts extracellular AMP to adenosine. When grown under normal conditions, these cells also express the A1, A2A, and A2B AR subtypes. Additionally, hCMEC/D3 cells are responsive to extracellular AR signaling, as cAMP levels increase following the addition of the broad spectrum AR agonist 5'-N-ethylcarboxamidoadenosine (NECA). Overall, these results indicate that human brain endothelial cells, and most likely the human BBB, have the capacity to synthesize and respond to extracellular adenosine.

Item Type: Journal Article
Copyright Holders: 2011 Springer Science+Business Media B.V.
ISSN: 1573-9546
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetNational Institutes of Health grant: R01 NS063011
Keywords: human brain endothelial cells; blood brain barrier; hCMEC/D3 cells; adenosine receptors; CD73
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 29828
Depositing User: Ignacio A Romero
Date Deposited: 19 Oct 2011 16:17
Last Modified: 07 Mar 2014 15:06
URI: http://oro.open.ac.uk/id/eprint/29828
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