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Insights into the putative catechin and epicatechin transport across blood-brain barrier

Faria, Ana; Pestana, Diogo; Teixeira, Diana; Couraud, Pierre-Olivier; Romero, Ignacio A; Weksler, Babette; de Freitas, Victor; Mateus, Nuno and Calhau, Conceição (2011). Insights into the putative catechin and epicatechin transport across blood-brain barrier. Food & Function(2) pp. 39–44.

URL: http://www.rsc.org/foodfunction
DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1039/c0fo00100g
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Abstract

The identification of mechanisms associated with phenolic neuroprotection is delayed due to a lack of information regarding the ability of phenolic compounds to enter the central nervous system (CNS). The aim of this work was to evaluate the transmembrane transport of catechin and epicatechin across blood-brain barrier (BBB). Two BBB cell lines, RBE-4 cells (immortalized cell line of rat capillary cerebral endothelial cells) and hCMEC/D3 (immortalized human cerebral microvessel endothelial cell line), were used. HPLC-DAD/MS was used to detect these compounds and their metabolites in the studied samples. The metabolites of the tested flavan-3-ols were synthesized to be used as standards. Catechin and epicatechin could cross both cells in a time-dependent manner. This transport was stereoselective (epicatechin[catechin), involving one or more stereoselective entities. Additionally, these cells were capable of metabolizing these compounds, particularly by conjugation with glucuronic acid, since this metabolite was detected in the basolateral media. Several studies suggest that blood levels of catechin and epicatechin are far below the levels used in this study and that these compounds appeared mainly as methyl, sulfate and glucuronide metabolites. Nevertheless, the information obtained by this study is valuable for the new insights about flavan-3-ols transport. In conclusion: (i) catechin and epicatechin are capable of crossing the BBB; (ii) a stereoselective process was involved in the passage of these compounds across BBB cells; (iii) these endothelial cells have enzymes capable of metabolizing these compounds.

Item Type: Journal Article
Copyright Holders: 2011 The Royal Society of Chemistry
ISSN: 2042-650X
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 29825
Depositing User: Ignacio A Romero
Date Deposited: 19 Oct 2011 15:35
Last Modified: 07 Mar 2014 15:06
URI: http://oro.open.ac.uk/id/eprint/29825
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