Michaluk, Piotr; Wawrzyniak, Marcin; Alot, Przemyslaw; Szczot, Marcin; Wyrembek, Paulina; Mercik, Katarzyna; Medvedev, Nikolay; Wilczek, Ewa; De Roo, Mathias; Zuschratter, Werner; Muller, Dominique; Wilczynski, Grzegorz M; Mozrzymas, Jerzy W; Stewart, Michael G.; Kaczmarek, Leszek and Wlodarczyk, Jakub
Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology.
Journal of cell science, 124(Pt 19),
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An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.
||2011. Published by The Company of Biologists Ltd
||The Polish Ministry of Science and Higher Education research grant P-N/030/2006 (J.W.M. and L.K.), 7th FP EU grant Memstick (M.G.S. and L.K.), Polish–Norwegian Research Fund grant (PNRF-96, G.M.W. and L.K.)
||brain; extracellular matrix; plasticity; proteases; MMP-9
||Science > Life, Health and Chemical Sciences
||29 Sep 2011 09:56
||16 Nov 2012 14:07
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