Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology

Michaluk, Piotr; Wawrzyniak, Marcin; Alot, Przemyslaw; Szczot, Marcin; Wyrembek, Paulina; Mercik, Katarzyna; Medvedev, Nikolay; Wilczek, Ewa; De Roo, Mathias; Zuschratter, Werner; Muller, Dominique; Wilczynski, Grzegorz M; Mozrzymas, Jerzy W; Stewart, Michael G.; Kaczmarek, Leszek and Wlodarczyk, Jakub (2011). Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. Journal of cell science, 124(Pt 19) pp. 3369–3380.

DOI (Digital Object Identifier) Link:	http://dx.doi.org/10.1242/jcs.090852

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Abstract

An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn²⁺-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.

Item Type:

Journal Article

2011. Published by The Company of Biologists Ltd

ISSN:

1477-9137

Project Funding Details:

Funded Project Name	Project ID	Funding Body
Not Set	P-N/030/2006	The Polish Ministry of Science and Higher Education
Not Set	7th FP EU grant Memstick	European Union
Not Set	PNRF-96	Polish–Norwegian Research Fund
Not Set	Not Set	Foundation for Polish Science
Not Set	Not Set	European Molecular Biology Organization
Not Set	N401 541540	Polish Ministry for Science