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Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology

Michaluk, Piotr; Wawrzyniak, Marcin; Alot, Przemyslaw; Szczot, Marcin; Wyrembek, Paulina; Mercik, Katarzyna; Medvedev, Nikolay; Wilczek, Ewa; De Roo, Mathias; Zuschratter, Werner; Muller, Dominique; Wilczynski, Grzegorz M; Mozrzymas, Jerzy W; Stewart, Michael G.; Kaczmarek, Leszek and Wlodarczyk, Jakub (2011). Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. Journal of cell science, 124(Pt 19) pp. 3369–3380.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1242/jcs.090852
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Abstract

An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.

Item Type: Journal Article
Copyright Holders: 2011. Published by The Company of Biologists Ltd
ISSN: 1477-9137
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetP-N/030/2006 The Polish Ministry of Science and Higher Education
Not Set7th FP EU grant Memstick European Union
Not SetPNRF-96Polish–Norwegian Research Fund
Not SetNot SetFoundation for Polish Science
Not SetNot SetEuropean Molecular Biology Organization
Not SetN401 541540Polish Ministry for Science
Keywords: brain; extracellular matrix; plasticity; proteases; MMP-9
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Related URLs:
Item ID: 29598
Depositing User: Michael Stewart
Date Deposited: 29 Sep 2011 09:56
Last Modified: 07 Mar 2014 15:05
URI: http://oro.open.ac.uk/id/eprint/29598
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