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Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and α-skeletal actin

Golding, Jon P.; Partridge, Terence A.; Beauchamp, Jonathan R.; King, Tim; Brown, Nigel A.; Gassmann, Martin and Zammit, Peter S. (2004). Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and α-skeletal actin. Developmental Dynamics, 231(4) pp. 795–800.

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Most muscle originates from the myotomal compartment of the somites, paired structures flanking the neural tube. Whereas vertebrate embryos show molecular and morphological asymmetry about the left-right body axis, somitic myogenesis is thought to occur symmetrically. Here, we provide the first evidence that myotome pairs are transiently left-right asymmetric, with higher expression of α-skeletal actin and myosin light chain 3F (MLC3F) on the left side between embryonic day 9.5-10.25. In iv mutants with situs inversus, the asymmetric expression of α-skeletal actin and MLC3F was inverted, showing that this process is regulated by global left-right axis cues, initiated before gastrulation. However, although left-sided identity is later maintained by Pitx2 genes, we found that Pitx2c null embryos have normal left-biased expression of α-skeletal actin and MLC3F. Myotome asymmetry, therefore, is downstream of the iv mutation but upstream of, or unrelated to, the Pitx2c pathway.

Item Type: Journal Article
Copyright Holders: 2004 Wiley-Liss, Inc.
ISSN: 1097-0177
Extra Information: Some of the symbols may not have transferred correctly into this bibliographic record and/or abstract.
Keywords: myotome; embryogenesis; left-right asymmetry; differentiation; iv mutation; Pitx2; alpha-SA; MLC3F
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 2781
Depositing User: Jon Golding
Date Deposited: 28 Jun 2006
Last Modified: 14 Jan 2016 15:51
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