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Golding, Jon P.; Partridge, Terence A.; Beauchamp, Jonathan R.; King, Tim; Brown, Nigel A.; Gassmann, Martin and Zammit, Peter S.
(2004).
DOI: https://doi.org/10.1002/dvdy.20176
Abstract
Most muscle originates from the myotomal compartment of the somites, paired structures flanking the neural tube. Whereas vertebrate embryos show molecular and morphological asymmetry about the left-right body axis, somitic myogenesis is thought to occur symmetrically. Here, we provide the first evidence that myotome pairs are transiently left-right asymmetric, with higher expression of α-skeletal actin and myosin light chain 3F (MLC3F) on the left side between embryonic day 9.5-10.25. In iv mutants with situs inversus, the asymmetric expression of α-skeletal actin and MLC3F was inverted, showing that this process is regulated by global left-right axis cues, initiated before gastrulation. However, although left-sided identity is later maintained by Pitx2 genes, we found that Pitx2c null embryos have normal left-biased expression of α-skeletal actin and MLC3F. Myotome asymmetry, therefore, is downstream of the iv mutation but upstream of, or unrelated to, the Pitx2c pathway.
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About
- Item ORO ID
- 2781
- Item Type
- Journal Item
- ISSN
- 1097-0177
- Extra Information
- Some of the symbols may not have transferred correctly into this bibliographic record and/or abstract.
- Keywords
- myotome; embryogenesis; left-right asymmetry; differentiation; iv mutation; Pitx2; alpha-SA; MLC3F
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 2004 Wiley-Liss, Inc.
- Depositing User
- Jon Golding