Golding, Jon P.; Partridge, Terence A.; Beauchamp, Jonathan R.; King, Tim; Brown, Nigel A.; Gassmann, Martin and Zammit, Peter S.
|DOI (Digital Object Identifier) Link:||http://dx.doi.org/10.1002/dvdy.20176|
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Most muscle originates from the myotomal compartment of the somites, paired structures flanking the neural tube. Whereas vertebrate embryos show molecular and morphological asymmetry about the left-right body axis, somitic myogenesis is thought to occur symmetrically. Here, we provide the first evidence that myotome pairs are transiently left-right asymmetric, with higher expression of α-skeletal actin and myosin light chain 3F (MLC3F) on the left side between embryonic day 9.5-10.25. In iv mutants with situs inversus, the asymmetric expression of α-skeletal actin and MLC3F was inverted, showing that this process is regulated by global left-right axis cues, initiated before gastrulation. However, although left-sided identity is later maintained by Pitx2 genes, we found that Pitx2c null embryos have normal left-biased expression of α-skeletal actin and MLC3F. Myotome asymmetry, therefore, is downstream of the iv mutation but upstream of, or unrelated to, the Pitx2c pathway.
|Item Type:||Journal Article|
|Copyright Holders:||2004 Wiley-Liss, Inc.|
|Extra Information:||Some of the symbols may not have transferred correctly into this bibliographic record and/or abstract.|
|Keywords:||myotome; embryogenesis; left-right asymmetry; differentiation; iv mutation; Pitx2; alpha-SA; MLC3F|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Jon Golding|
|Date Deposited:||28 Jun 2006|
|Last Modified:||14 Jan 2016 15:51|
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