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Myelin basic protein content of aggregating rat brain cell cultures treated with cytokines and/or demyelinating antibody: effects of macrophage enrichment

Loughlin, A. J.; Woodroofe, M. N.; Cuzner, M. L.; Honegger, P.; Comte, V. and Matthieu, J. M. (1994). Myelin basic protein content of aggregating rat brain cell cultures treated with cytokines and/or demyelinating antibody: effects of macrophage enrichment. Journal of neuroscience research, 37(5) pp. 647–653.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1002/jnr.490370512
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Abstract

The demyelinative potential of the cytokines interleukin-1 alpha (IL-1 alpha), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) has been investigated in myelinating aggregate brain cell cultures. Treatment of myelinated cultures with these cytokines resulted in a reduction in myelin basic protein (MBP) content. This effect was additively increased by anti-myelin/oligodendrocyte glycoprotein (alpha-MOG) in the presence of complement. Qualitative immunocytochemistry demonstrated that peritoneal macrophages, added to the fetal telencephalon cell suspensions at the start of the culture period, successfully integrated into aggregate cultures. Supplementing the macrophage component of the cultures in this fashion resulted in increased accumulation of MBP. The effect of IFN-gamma on MBP content of cultures was not affected by the presence of macrophages in increased numbers.

Item Type: Journal Article
Copyright Holders: 1994 Wiley-Liss, Inc.
ISSN: 1097-4547
Keywords: myelination; demyelination;macrophages; cytokines; myelin basic protein; multiple sclerosis
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 27029
Depositing User: Jane Loughlin
Date Deposited: 20 Apr 2011 10:04
Last Modified: 07 Mar 2014 13:47
URI: http://oro.open.ac.uk/id/eprint/27029
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