Loughlin, A. J.; Woodroofe, M. N.; Cuzner, M. L.; Honegger, P.; Comte, V. and Matthieu, J. M.
|DOI (Digital Object Identifier) Link:||http://dx.doi.org/10.1002/jnr.490370512|
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The demyelinative potential of the cytokines interleukin-1 alpha (IL-1 alpha), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) has been investigated in myelinating aggregate brain cell cultures. Treatment of myelinated cultures with these cytokines resulted in a reduction in myelin basic protein (MBP) content. This effect was additively increased by anti-myelin/oligodendrocyte glycoprotein (alpha-MOG) in the presence of complement. Qualitative immunocytochemistry demonstrated that peritoneal macrophages, added to the fetal telencephalon cell suspensions at the start of the culture period, successfully integrated into aggregate cultures. Supplementing the macrophage component of the cultures in this fashion resulted in increased accumulation of MBP. The effect of IFN-gamma on MBP content of cultures was not affected by the presence of macrophages in increased numbers.
|Item Type:||Journal Article|
|Copyright Holders:||1994 Wiley-Liss, Inc.|
|Keywords:||myelination; demyelination;macrophages; cytokines; myelin basic protein; multiple sclerosis|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Jane Loughlin|
|Date Deposited:||20 Apr 2011 10:04|
|Last Modified:||15 Jan 2016 17:02|
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