Loughlin, A. J. and Woodroofe, M. N.
(1996).
Inhibitory effect of interferon-gamma on LPS-induced interleukin 1 beta production by isolated adult rat brain microglia.
Neurochemistry International, 29(1),
pp. 77–82.
Abstract
The effect of interferon-γ (IFN-γ) on lipopolysaccharide (LPS)-induced cytokine production has been examined in adult rat brain microglia. Following treatment of isolated cells in vitro with LPS, interleukin-1 (IL-1) and interleukin-6 (IL-6) levels in culture supernatants, determined by bioassay, were increased in a dose-dependent manner. IL-6 was particularly sensitive to LPS-stimulation. Using in situ hybridisation techniques, the induction by LPS of IL-1 and IL-6 mRNA in cultured microglia has been demonstrated. When IFN-γ was included, LPS-induced production of both IL-1 and IL-6 by microglia was significantly reduced and this effect was particularly marked in the case of IL-1. Comparisons with peritoneal macrophages demonstrate that whilst both cell types produce IL-1 and IL-6 in response to LPS, IFN-γ had no significant effect on this response in peritoneal macrophages. This suggests potential control mechanisms which may be effective in down-regulating cytokine production by microglia in vivo
| Item Type: |
Journal Article
|
| Copyright Holders: |
1996 Elsevier Science Ltd |
| ISSN: |
0197-0186 |
| Funders: |
the Multiple Sclerosis Society of Great Britain and Northern Ireland, the Brain Research Trust |
| Academic Unit/Department: |
Science > Life, Health and Chemical Sciences |
| Item ID: |
27026 |
| Depositing User: |
Jane Loughlin
|
| Date Deposited: |
20 Apr 2011 10:53 |
| Last Modified: |
20 Apr 2011 10:53 |
| URI: |
http://oro.open.ac.uk/id/eprint/27026 |
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