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Inhibitory effect of interferon-gamma on LPS-induced interleukin 1 beta production by isolated adult rat brain microglia

Loughlin, A. J. and Woodroofe, M. N. (1996). Inhibitory effect of interferon-gamma on LPS-induced interleukin 1 beta production by isolated adult rat brain microglia. Neurochemistry International, 29(1) pp. 77–82.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1016/0197-0186(95)00135-2
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Abstract

The effect of interferon-γ (IFN-γ) on lipopolysaccharide (LPS)-induced cytokine production has been examined in adult rat brain microglia. Following treatment of isolated cells in vitro with LPS, interleukin-1 (IL-1) and interleukin-6 (IL-6) levels in culture supernatants, determined by bioassay, were increased in a dose-dependent manner. IL-6 was particularly sensitive to LPS-stimulation. Using in situ hybridisation techniques, the induction by LPS of IL-1 and IL-6 mRNA in cultured microglia has been demonstrated. When IFN-γ was included, LPS-induced production of both IL-1 and IL-6 by microglia was significantly reduced and this effect was particularly marked in the case of IL-1. Comparisons with peritoneal macrophages demonstrate that whilst both cell types produce IL-1 and IL-6 in response to LPS, IFN-γ had no significant effect on this response in peritoneal macrophages. This suggests potential control mechanisms which may be effective in down-regulating cytokine production by microglia in vivo

Item Type: Journal Article
Copyright Holders: 1996 Elsevier Science Ltd
ISSN: 0197-0186
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot Setthe Multiple Sclerosis Society of Great Britain and Northern Ireland
Not SetNot Setthe Brain Research Trust
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 27026
Depositing User: Jane Loughlin
Date Deposited: 20 Apr 2011 10:53
Last Modified: 07 Mar 2014 13:47
URI: http://oro.open.ac.uk/id/eprint/27026
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