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Myelination and remyelination of aggregate rat brain cell cultures enriched with macrophages

Loughlin, A. J.; Copelman, C. A.; Hall, A.; Armer, T.; Young, B. C.; Landon, D. N. and Cuzner, M. L. (1997). Myelination and remyelination of aggregate rat brain cell cultures enriched with macrophages. Journal of neuroscience research, 47(4) pp. 384–392.

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We reported previously that accumulation of myelin basic protein (MBP) in foetal brain aggregate cultures is enhanced by supplementation with peritoneal macrophages. The present study demonstrates that the rate of MBP accumulation in macrophage-enriched cultures continues to increase over time unaccompanied by a matching increase in the oligodendrocyte marker cyclic nucleotide phosphodiesterase, while that of control cultures reaches a plateau. These observations are supported by electron microscopic evidence of cumulative numbers of myelinated axons in the aggregates over time and by enhanced expression of myelin protein genes in macrophage-enriched relative to control cultures. Aggregates demyelinate following short-term exposure to cytokines and anti-myelin oligodendrocyte glycoprotein antibody, and MBP synthesis resumes following removal of demyelinating agents. Supplementation of cultures with macrophages influences the degree of myelin breakdown and remyelination, drawing attention to the role that macrophage-derived growth factors may play in myelinogenesis and myelin repair in inflammatory demyelinating disease.

Item Type: Journal Item
Copyright Holders: 1997 Wiley-Liss Inc.
ISSN: 1097-4547
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetAIMS (Northwest)
Not SetNot Setthe Multiple Sclerosis Society of Great Britain and Northern Ireland
Keywords: myelination; remyelination; macrophages
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 27024
Depositing User: Jane Loughlin
Date Deposited: 20 Apr 2011 10:19
Last Modified: 07 Dec 2018 09:50
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