Rapid qualitative and quantitative analysis of opiates in extract of poppy head via FTIR and chemometrics: towards in-field sensors

Turner, Nicholas W.; Cauchi, Michael; Piletska, Elena V.; Preston, Christopher and Piletsky, Sergey A. (2009). Rapid qualitative and quantitative analysis of opiates in extract of poppy head via FTIR and chemometrics: towards in-field sensors. Biosensors and Bioelectronics, 24(11) pp. 3322–3328.

DOI: https://doi.org/10.1016/j.bios.2009.04.030

Abstract

Identification and quantification of the opiates morphine and thebaine has been achieved in three commercial poppy cultivars using FTIR-ATR spectroscopy, from a simple and rapid methanolic extraction, suitable for field analysis. The limits of detection were 0.13 mg/ml (0.013%, w/v) and 0.3 mg/ml (0.03%, w/v) respectively. The concentrations of opiates present were verified with HPLC–MS. The chemometrics has been used to identify specific “signature” peaks in the poppy IR spectra for characterisation of cultivar by its unique fingerprint offering a potential forensic application in opiate crop analysis.

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