Study of methylation and ethylation by thermochemolysis of tuberculostearic acid from Mycobacterium tuberculosis culture and sputum

Nicoara, Simona C.; O'Sullivan, Denise O.; McNerney, Ruth; Corbet, Elizabeth L.; Mutetwa, Reggie; Minnikin, David and Morgan, Geraint H. (2010). Study of methylation and ethylation by thermochemolysis of tuberculostearic acid from Mycobacterium tuberculosis culture and sputum. In: The 26th Informal Meeting on Mass Spectrometry, 2-6 May 2010, Koszeg, Hungary.

Abstract

The direct analysis of TBSA was performed by thermally assisted hydrolysis and alkylation in M.tb culture dilutions and in sputum deposits, using tetramethyl ammonium hydroxide (TMAH) and tetraethylammonium hydroxide (TEAH) as derivatizing agents. Thermochemolysis of fatty acids was done in a programmable temperature PTV inlet Optic3, combined with an automatic LINEX liner exchanger installed on an Agilent GC-MS system. The GC-MS response is discussed and the results are compared for the two alkylating reagents used.

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About

  • Item ORO ID
  • 26714
  • Item Type
  • Conference or Workshop Item
  • Keywords
  • M. tuberculosis; tuberculostearic acid; online derivatization of fatty acids; gas chromatography; mass spectrometry
  • Academic Unit or School
  • Faculty of Science, Technology, Engineering and Mathematics (STEM)
  • Copyright Holders
  • © 2010 The Authors
  • Depositing User
  • Simona Nicoarǎ

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