Agostinho, Raquel Terra; David Santos-Filho, S; De Souza da Fonseca, A; Missailidis, Sotiris and Bernardo-Filho, M
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This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi extract altered significantly (p<0.05) the %ATI of P, BC, IF-P, SF-P, IF-BC and SF-BC, as well as increased the survival of bacterial cultures treated with stannous chloride. Our results suggest that reishi extract could present a redox/chelating action, altering the labeling of blood constituents with 99mTc and protecting bacterial cultures against oxidative damage induced by stannous chloride.
|Item Type:||Journal Article|
|Copyright Holders:||2008 Brazilian Archives of Biology and Technology|
|Keywords:||blood constituents; Escherichia coli; Ganoderma lucidum; stannous chloride; technetium-99m|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Interdisciplinary Research Centre:||Biomedical Research Network (BRN)|
|Depositing User:||Sotiris Missailidis|
|Date Deposited:||10 Jan 2011 09:39|
|Last Modified:||23 Feb 2016 21:07|
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