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Effects of urethane anaesthesia on sensory processing in the rat barrel cortex revealed by combined optical imaging and electrophysiology

Devonshire, Ian M.; Grandy, Thomas H.; Dommett, Eleanor J. and Greenfield, Susan A. (2010). Effects of urethane anaesthesia on sensory processing in the rat barrel cortex revealed by combined optical imaging and electrophysiology. European Journal of Neuroscience, 32(5) pp. 786–97.

DOI (Digital Object Identifier) Link: http://dx.doi.org/10.1111/j.1460-9568.2010.07322.x
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Abstract

The spatiotemporal dynamics of neuronal assemblies evoked by sensory stimuli have not yet been fully characterised, especially the extent to which they are modulated by prevailing brain states. In order to examine this issue, we induced different levels of anaesthesia, distinguished by specific electroencephalographic indices, and compared somatosensory-evoked potentials (SEPs) with voltage-sensitive dye imaging (VSDI) responses in the rat barrel cortex evoked by whisker deflection. At deeper levels of anaesthesia, all responses were reduced in amplitude but, surprisingly, only VSDI responses exhibited prolonged activation resulting in a delayed return to baseline. Further analysis of the optical signal demonstrated that the reduction in response amplitude was constant across the area of activation, resulting in a global down-scaling of the population response. The manner in which the optical signal relates to the various neuronal generators that produce the SEP signal is also discussed. These data provide information regarding the impact of anaesthetic agents on the brain, and show the value of combining spatial analyses from neuroimaging approaches with more traditional electrophysiological techniques.

Item Type: Journal Article
Copyright Holders: 2010 University of Oxford. European Journal of Neuroscience, 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
ISSN: 0953-816X
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetJames Martin Foundation
Keywords: rodent; somatosensory; voltage-sensitive dye imaging
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 24722
Depositing User: Ellie Dommett
Date Deposited: 30 Dec 2010 14:15
Last Modified: 07 Mar 2014 13:42
URI: http://oro.open.ac.uk/id/eprint/24722
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