VUV irradiation studies of plasmid DNA in aqueous solution

Smialek, M. A.; Hoffman, S. V.; Folkard, M.; Prise, K. M.; Shuker, D. E. G.; Braithwaite, N. St. J. and Mason, N. J. (2008). VUV irradiation studies of plasmid DNA in aqueous solution. In: Radiation Damage in Biomolecular Systems (McGuigan, KG ed.), 19-22 Jul 2007, Dublin, p. 12020.




Interactions of VUV light and DNA samples in aqueous solutions are reported. The damage induced by such radiation is quantified by monitoring both loss of supercoiled DNA and formation of single and double strand breaks using agarose gel electrophoresis. Irradiations were performed using synchrotron VUV photons of 130, 150, 170 and 190 nm. VUV irradiation experiments revealed enhanced damage upon irradiation with 170 nm photons as compared with irradiations with photons of 150 nm and 130 nm. Irradiations carried at 190 nm caused the least damage.

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