Peddie, C. J.; Davies, H. A.; Colyer, F M.; Stewart, M. G. and Rodríguez, J. J.
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|DOI (Digital Object Identifier) Link:||http://dx.doi.org/doi:10.1016/j.neulet.2010.09.024|
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The serotonin1B receptor (5-HT1BR) plays a role in cognitive processes that also involve glutamatergic neurotransmission via amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptors. Accumulating experimental evidence also highlights the involvement of 5-HT1BRs in several neurological disorders. Consequently, the 5-HT1BR is increasingly implicated as a potential therapeutic target for intervention in cognitive dysfunction. Within the hippocampus, a brain region critical to cognitive processing, populations of pre- and post-synaptic 5-HT1BRs have been identified. Thus, 5-HT<sub1B</sub>Rs could have a role in the modulation of hippocampal pre- and post-synaptic conductance. Previously, we demonstrated colocalization of 5-HT1BRs with the N-methyl-d-aspartate (NMDA) receptor subunit NR1 in a subpopulation of granule cell dendrites (Peddie et al. ). In this study, we have examined the cellular and subcellular distribution of 5-HT1BRs with the AMPA receptor subunit GluR2. Of 5-HT1BR positive profiles, 28% displayed colocalization with GluR2. Of these, 87% were dendrites, corresponding to 41% and 10% of all 5-HT1BR labeled or GluR2 labeled dendrites, respectively. Dendritic labeling was both cytoplasmic and membranous but was not usually associated with synaptic sites. Colocalization within dendritic spines and axons was comparatively rare. These findings indicate that within the dentate gyrus molecular layer, dendritic 5-HT1BRs are expressed predominantly on GluR2 negative granule cell processes. However, a subpopulation of 5-HT1BRs is expressed on GluR2 positive dendrites. Here, it is suggested that activation of the 5-HT1BR may play a role in the modulation of AMPA receptor mediated conductance, further supporting the notion that the 5-HT1BR represents an interesting therapeutic target for modulation of cognitive function.
|Item Type:||Journal Article|
|Copyright Holders:||2010 Elsevier Ireland Ltd|
|Keywords:||serotonin; hippocampus; glutamate; localization; ultrastructure; 5-HT1BR; GluR2; receptor|
|Academic Unit/Department:||Science > Life, Health and Chemical Sciences
|Depositing User:||Astrid Peterkin|
|Date Deposited:||01 Nov 2010 12:18|
|Last Modified:||26 Oct 2012 11:22|
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